TY - JOUR

T1 - Application of a novel sol-gel polydimethylsiloxane-poly(vinyl alcohol) solid-phase microextraction fiber for gas chromatographic determination of pesticide residues in herbal infusions

AU - Zuin, Vânia Gomes

AU - Lopes, Alexandre Leite

AU - Yariwake, Janete Harumi

AU - Augusto, Fabio

N1 - Funding Information: The authors thank Dr. Ana M. S. Pereira (UNAERP) and Maria C. H. Reimberg (Centroflora-Anidro) for providing referential plant material, Gilmare A. Silva and Reinaldo Teófilo (IQ-UNICAMP) for help with the multivariate SPME study and the Brazilian research funding institutions FAPESP, CAPES and CNPq for their financial support and scholarships.

PY - 2004/11/12

Y1 - 2004/11/12

N2 - A simple and environmentally friendly methodology for headspace solid-phase microextraction (HS-SPME) using a new fiber coated with polydimethylsiloxane- poly(vinyl alcohol) (PDMS/PVA) is reported for the trace determination of organochlorine (OCP) and organophosphorus (OPP) pesticides in herbal infusions of Passiflora L. by GC-ECD. The capacity of the PDMS/PVA coating for the pesticides was compared to that of commercial PDMS fibers, with advantageous results. The effects of parameters such as the sample ionic strength, dilution of the infusion, extraction temperature and time were investigated. The optimized conditions for the determination of OCP and OPP in Passiflora L. infusions were extraction time and temperature, respectively, of 38 min and 67.5°C, with 5 min of sample/headspace equilibration time. The analytical curves for the range between 0.04 ng mL -1 to 6 ng mL -1 of each compound presented a good quality (correlation coefficients of 0.921 or better). The detection limits for the OCP and OPP in these matrices varied from 0.01 ng mL -1 (β-endosulfan) to 1.5 ng mL -1 (malathion). The sensitivity of studied methodology was adequate, as well as its accuracy (78.7-91.5%) and precision (R.S.D. = 1.2-14.2%).

AB - A simple and environmentally friendly methodology for headspace solid-phase microextraction (HS-SPME) using a new fiber coated with polydimethylsiloxane- poly(vinyl alcohol) (PDMS/PVA) is reported for the trace determination of organochlorine (OCP) and organophosphorus (OPP) pesticides in herbal infusions of Passiflora L. by GC-ECD. The capacity of the PDMS/PVA coating for the pesticides was compared to that of commercial PDMS fibers, with advantageous results. The effects of parameters such as the sample ionic strength, dilution of the infusion, extraction temperature and time were investigated. The optimized conditions for the determination of OCP and OPP in Passiflora L. infusions were extraction time and temperature, respectively, of 38 min and 67.5°C, with 5 min of sample/headspace equilibration time. The analytical curves for the range between 0.04 ng mL -1 to 6 ng mL -1 of each compound presented a good quality (correlation coefficients of 0.921 or better). The detection limits for the OCP and OPP in these matrices varied from 0.01 ng mL -1 (β-endosulfan) to 1.5 ng mL -1 (malathion). The sensitivity of studied methodology was adequate, as well as its accuracy (78.7-91.5%) and precision (R.S.D. = 1.2-14.2%).

KW - Passiflora L.

KW - Pesticides

KW - Phytomedicines

KW - Chemistry

UR - http://www.scopus.com/inward/record.url?scp=8644253629&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2004.07.074

DO - 10.1016/j.chroma.2004.07.074

M3 - Journal articles

C2 - 15595528

AN - SCOPUS:8644253629

VL - 1056

SP - 21

EP - 26

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1-2 SPEC.ISS.

ER -