Despite the normative nature of sustainability, values and their role in sustainability transformations are often discussed in vague terms, and when concrete conceptualizations exist, they widely differ across fields of application. To provide guidance for navigating the complexity arising from the various conceptualizations and operationalization of values, here, we differentiate four general perspectives of how and where values are important for transformation related sustainability science. The first perspective, surfacing implicit values, revolves around critical reflection on normative assumptions in scientific practices. Sustainability transformations concern fundamental ethical questions and are unavoidably influenced by assumptions sustainability scientists hold in their interactions with society. The second perspective, negotiating values, is related to the values held by different actors in group decision processes. Developing and implementing solution options to sustainability problems requires multiple values to be accounted for in order to increase civic participation and social legitimacy. The third perspective, eliciting values, focuses on the ascription of values to particular objects or choices related to specific sustainability challenges, for example, valuations of nature. The fourth perspective, transforming through values, highlights the dynamic nature and transformational potential of values. Value change is complex but possible, and may generate systemic shifts in patterns of human behaviours. Explicit recognition of these four interconnected values perspectives can help sustainability scientists to: (1) move beyond general discussions implying that values matter; (2) gain an awareness of the positionality of one’s own values perspective when undertaking values related sustainability research; and (3) reflect on the operationalizations of values in different contexts.