Toxicity testing with luminescent bacteria - Characterization of an automated method for the combined assessment of acute and chronic effects

Research output: Journal contributionsJournal articlesResearchpeer-review

Authors

The luminescent bacteria test according to EN ISO 11348 is frequently applied in (eco) toxicity testing and is applicable for a huge variety of environmental and industrial samples. A big disadvantage of this method is the very short exposure time, which is expressed in a low sensitivity in regard to substances with a delayed effect. Chronic effects, i.e. interference with cell growth, cannot be assessed with this conventional standard method. The goal of this research was to develop an automated testing system for long term toxicity towards the luminescent bacteria Vibrio fischeri by implementing microtitration-based instrumentation. The optimized method, hereinafter referred to as "kinetic luminescent bacteria test", can be described as a miniaturized combination of the conventional short-term luminescence inhibition test according to EN ISO 11348 and the Photobacterium phosphoreum growth inhibition test (DIN 38412-37). The validation procedure included the evaluation of six reference compounds (3,4-Dichloroaniline, 3,5-Dichlorophenol, Chloramphenicol, Streptomycin sulfate, Potassium dichromate, Zinc sulfate heptahydrate) and three different endpoints that are acute luminescence inhibition (acute LI) after 30 min, chronic luminescence inhibition (chronic LI) after 24 h and growth inhibition (GI) after 14 h. The optimized method allows the assessment of acute and chronic effects within one test, by what a misinterpretation of the toxicity of substances with delayed bacterial toxicity can be prevented, without abandoning most of the advantages of the conventional short-term test. Therefore, the kinetic luminescent bacteria test is exceptional as an initial screening test for environmental samples or substances with unknown (eco) toxicological characteristics.
Original languageEnglish
JournalChemosphere
Volume93
Issue number6
Pages (from-to)990-996
Number of pages7
ISSN0045-6535
DOIs
Publication statusPublished - 10.2013

    Research areas

  • Chemistry - Chronic toxicity, Ecotoxicity, Environmental risk assessment, Growth inhibition, Luminescence inhibition, Vibrio fischeri
  • Biology

Recently viewed

Researchers

  1. Mathis Brinkmann

Publications

  1. Towards more effective and transferable transition experiments
  2. Exploring Difficult History Lessons, Identity Construction, the Artistic Expansion of Sitcom Storytelling Tools in the Black-ish Episode, "Juneteenth"
  3. Computational Study of Three-Dimensional Lagrangian Transport and Mixing in a Stirred Tank Reactor  
  4. Quantification of phototrophically grown Galdieria sulphuraria and other microalgae using diphenylamine
  5. Principals between exploitation and exploration
  6. Global Integration and Management of Professional Service Firms
  7. Degradation of 5-FU by means of advanced (photo)oxidation processes
  8. “You’re Not Allowed to Give Us the Solution, but Can You Guide Us towards It?”
  9. Does it occur or not? - A structured approach to support students in determining the spontaneity of chemical reactions
  10. Clusteranalyse als Methode zur Strukturierung großer Datenmodelle
  11. Datenkritik
  12. The contralateral effects of foam rolling on range of motion and muscle performance
  13. Data practices in apps from Brazil: What do privacy policies inform us about?
  14. Dynamic performance
  15. Accuracy Improvement by Artificial Neural Networks in Technical Vision System
  16. What has gone wrong with application development? Who is the culprit?
  17. Can isometric testing substitute for the one repetition maximum squat test?
  18. Adjusting the Sails
  19. Development and application of green and sustainable analytical methods for flavonoid extraction from Passiflora waste
  20. Why the future is democratic
  21. Integrated driver rostering problem in public bus transit
  22. Effects of oral corrective feedback on the development of complex morphosyntax
  23. The promise and challenges of computer mouse trajectories in DMHIs – A feasibility study on pre-treatment dropout predictions
  24. Der "fachdidaktische Code" der Lebenswelt- und/oder (?) Situationsorientierung
  25. Klimapaket
  26. Motivation and emotion as mediators in multimedia learning
  27. Jointly experimenting for transformation?
  28. The most frequent phrasal verbs in English language EU documents - A corpus-based analysis and its implications
  29. Managing information in the case of opinion spamming
  30. What do employers pay for employees’ complex problem solving skills?
  31. Perfluorooctanoic acid (PFOA)-main concerns and regulatory developments in Europe from an environmental point of view
  32. Construct Clean-Up in Proactivity Research
  33. Development and testing of the insulin treatment experience questionnaire (ITEQ)
  34. INVENTORY REDUCTION BY MODERN TECHNIQUES OF DYNAMIC REPLENISHMENT OF MATERIALS AVAILABILITY IN MANUFACTURING - PRACTICAL EXAMPLE FROM THE AUTOMOTIVE INDUSTRY
  35. Contributions of Net-Map to sustainability action research