Targeted metabolomics of pellicle and saliva in children with different caries activity

Research output: Journal contributionsJournal articlesResearchpeer-review

Authors

Pellicle is the initial proteinaceous layer that is formed almost instantaneously on all solid surfaces in the oral cavity. It is of essential relevance for any interactions and metabolism on the tooth surface. Up to now, there is no information on the metabolome of this structure. Accordingly, the present study aims to characterise the metabolomic profile of in-situ pellicle in children with different caries activity for the first time in comparison to saliva. Small molecules such as carbohydrates, amino acids, organic acids, and fatty acids, putatively involved in the formation of caries were quantified using mass spectrometry (MS)-based techniques, such as (stable isotope dilution analysis)-ultra-performance liquid chromatography-tandem MS and gas chromatography/electron ionisation-MS. Pellicle and corresponding saliva samples were collected from caries-active, caries-free and caries-rehabilitated 4- to 6-year-old children. The most abundant analytes in pellicle were acetic acid (1.2–10.5 nmol/cm 2), propionic acid (0.1–8.5 nmol/cm 2), glycine (0.7–3.5 nmol/cm 2), serine (0.08–2.3 nmol/cm 2), galactose (galactose + mannose; 0.035–0.078 nmol/cm 2), lactose (0.002–0.086 nmol/cm 2), glucose (0.018–0.953 nmol/cm 2), palmitic acid (0.26–2.03 nmol/cm 2), and stearic acid (0.34–1.81 nmol/cm 2). Significant differences depending on caries activity were detected neither in saliva nor in the corresponding pellicle samples.

Original languageEnglish
Article number697
JournalScientific Reports
Volume10
Issue number1
Number of pages11
ISSN2045-2322
DOIs
Publication statusPublished - 01.12.2020

Bibliographical note

This study was supported by the German Research Foundation (DFGHA 5192/ 11-1, HA 2718/17-1, and HO 2116/15-1). We thank S. Pötschke, B. Hartmann, and S. Basche for exquisite technical assistance, Dr. O. Frank, Dr. J. Kreissl, M. Gigl, and R. Hammerl for quantitative nuclear magnetic resonance measurements, and all children and their parents for participation in the study.

Publisher Copyright:
© 2020, The Author(s).

    Research areas

  • Chemistry - Medicine, Dentistry, Biochemistry

Documents

DOI

Recently viewed

Activities

  1. High resolution assessment and modelling of suspended sediment in an agricultural catchment
  2. Organization Studies (Fachzeitschrift)
  3. 19. Nachwuchsworkshop der - DGPs 2022
  4. 39th EGOS Colloquium - EGOS 2023
  5. Improving skills. Evidence from secondary analysis of international surveys 2012
  6. Skype, Tandem and other Technical Catastrophes
  7. Referent zum Vorsorgeprinzip in EU-Freihandelsabkommen
  8. Shaping the Future, Participation & Coping with Complexity – Culture as blind spot and pivotal Perspective of ESD
  9. Universität Konstanz
  10. The Ta Moko as a Figure of Art History
  11. „Zum Zeitvertreib“
  12. The Rule of Law’s Conceptual Roots in the Eras of Absolutism and Enlightenment
  13. How harmonious and obsessive passion lead to entrepreneurial success: Unfolding the underlying process on a state level.
  14. Beyond Diversity – Festival Curators as Gatekeepers
  15. Gamification in ELT: Meeting the needs of all learners
  16. Workshop at the DFG-Summer Academy on Educational Measurement - 2007
  17. "Listen I‘m sorry. I didn‘t mean to offend you.": Conventions of language use across the Englishes (Universität Erfurt)
  18. Camera Obscura (Zeitschrift)
  19. Mathematics teacher students´ use of instructional videos.
  20. Floating Utopias
  21. Path dependence: friend and foe for decision-making in organizations
  22. Fachtagung Sucht 2020
  23. Academy of Management Journal (Fachzeitschrift)
  24. Challenging the functionality of audits: Examining the bureaucratization of risks of industrial accidents through the eyes of Franz Kafka, novelist and auditor
  25. Environmental and Resource Economics (Fachzeitschrift)
  26. Workshop zum Standard-Setting Sozialindex 2012
  27. Organization Studies (Fachzeitschrift)
  28. The International Congress on the Problem Solving in Mathematics, ProMath 2004.
  29. Werkstattgespräch
  30. Standards for Inclusion & ICT Readiness: Ensuring Inclusiveness and Connectivity for Older Persons
  31. AMMODI Virtual Roundtable: "Making African(ist) Migration Research Visible"
  32. SOLWODI-Fachtagung 2020
  33. Focusing Events and Changes in the Governance of Garment Global Production Networks: The Case of Rana Plaza