Reprint of: Improved cytotoxicity testing of magnesium materials

Research output: Journal contributionsJournal articlesResearchpeer-review


  • Janine Fischer
  • Daniel Pröfrock
  • Norbert Hort
  • Regine Willumeit
  • Frank Feyerabend

Metallic magnesium (Mg) and its alloys are highly suitable for medical applications as biocompatible and biodegradable implant materials. Magnesium has mechanical properties similar to bone, stimulates bone regeneration, is an essential non-toxic element for the human body and degrades completely within the body environment. In consequence, magnesium is a promising candidate as implant material for orthopaedic applications. Protocols using the guideline of current ISO standards should be carefully evaluated when applying them for the characterization of the cytotoxic potential of degradable magnesium materials. For as-cast material we recommend using 10 times more extraction medium than recommended by the ISO standards to obtain reasonable results for reliable cytotoxicity rankings of degradable materials in vitro. In addition primary isolated human osteoblasts or mesenchymal stem cells should be used to test magnesium materials.

Original languageEnglish
JournalMaterials Science and Engineering B: Solid-State Materials for Advanced Technology
Issue number20
Pages (from-to)1773-1777
Number of pages5
Publication statusPublished - 15.12.2011
Externally publishedYes

    Research areas

  • Cytotoxicity tests, Implants, In vitro, ISO standards, Magnesium, Osmolality
  • Engineering