Orientation-driven photosynthesized carbon belowground mediates intercropped peanut microbiota changes for pathogen resistance
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Orientation-driven photosynthesized carbon belowground mediates intercropped peanut microbiota changes for pathogen resistance. / Lu, Jumeng; Shen, Yi; He, Ganghui et al.
In: Plant and Soil, 28.09.2023.Research output: Journal contributions › Journal articles › Research › peer-review
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TY - JOUR
T1 - Orientation-driven photosynthesized carbon belowground mediates intercropped peanut microbiota changes for pathogen resistance
AU - Lu, Jumeng
AU - Shen, Yi
AU - He, Ganghui
AU - Li, Shiwen
AU - Kumar, Amit
AU - Sun, Bo
AU - Chen, Yan
N1 - Publisher Copyright: © 2023, The Author(s), under exclusive licence to Springer Nature Switzerland AG.
PY - 2023/9/28
Y1 - 2023/9/28
N2 - Background and aims: Traditional intercropping of tall and short crops often maintain productivity at the expense of the fitness of the short crop due to planting orientation. There is a need to understand how light interception as influenced by row orientation, affects the vertical allocation of photosynthesized carbon, and how this impacts the rhizosphere microbiota of short crops. This understanding would allow for the optimization of aboveground design to utilize the belowground microbiota for plant and soil health in diversified cropping systems. Methods: We manipulated the row orientation (east-west vs. north-south) of peanut and maize in a field and conducted simulated pot experiment where peanut plants were shaded. By using 13C tracer approach and DNA stable isotope probing (DNA-SIP) method, we quantified C allocation by peanuts in its rhizosphere including the rhizosphere microorganisms. Moreover, by combining high-throughput sequencing and bacterial cultivation, we evaluated photosynthesized carbon driven the change of rhizosphere microbial composition and its interaction for fungal pathogen resistance. Results: Field intercropping in the north-south orientation increased peanut photosynthetically active radiation to over two times compared to the east-west orientation. The higher light interception increased the relative abundance of photosynthesized carbon which selectively enriched the rhizosphere biomarker Burkholderia to effectively suppressed the pathogenic fungus Alternaria alstroemeriae. Conclusion: North-south row orientation of peanut and maize intercropping can enhance the allocation of photosynthesized carbon in peanut rhizosphere by changing the light interception. The more photosynthesized carbon triggers the reshape of rhizosphere microbiota and induce beneficial Burkholderia to antagonize peanut pathogen to optimize peanut health.
AB - Background and aims: Traditional intercropping of tall and short crops often maintain productivity at the expense of the fitness of the short crop due to planting orientation. There is a need to understand how light interception as influenced by row orientation, affects the vertical allocation of photosynthesized carbon, and how this impacts the rhizosphere microbiota of short crops. This understanding would allow for the optimization of aboveground design to utilize the belowground microbiota for plant and soil health in diversified cropping systems. Methods: We manipulated the row orientation (east-west vs. north-south) of peanut and maize in a field and conducted simulated pot experiment where peanut plants were shaded. By using 13C tracer approach and DNA stable isotope probing (DNA-SIP) method, we quantified C allocation by peanuts in its rhizosphere including the rhizosphere microorganisms. Moreover, by combining high-throughput sequencing and bacterial cultivation, we evaluated photosynthesized carbon driven the change of rhizosphere microbial composition and its interaction for fungal pathogen resistance. Results: Field intercropping in the north-south orientation increased peanut photosynthetically active radiation to over two times compared to the east-west orientation. The higher light interception increased the relative abundance of photosynthesized carbon which selectively enriched the rhizosphere biomarker Burkholderia to effectively suppressed the pathogenic fungus Alternaria alstroemeriae. Conclusion: North-south row orientation of peanut and maize intercropping can enhance the allocation of photosynthesized carbon in peanut rhizosphere by changing the light interception. The more photosynthesized carbon triggers the reshape of rhizosphere microbiota and induce beneficial Burkholderia to antagonize peanut pathogen to optimize peanut health.
KW - Burkholderia
KW - Pathogen defence
KW - Photosynthesized carbon assimilation
KW - Photosynthetically active radiation
KW - Plant growth promotion
KW - Rhizosphere microbial functioning
KW - Biology
UR - http://www.scopus.com/inward/record.url?scp=85173021282&partnerID=8YFLogxK
U2 - 10.21203/rs.3.rs-2538293/v1
DO - 10.21203/rs.3.rs-2538293/v1
M3 - Journal articles
AN - SCOPUS:85173021282
JO - Plant and Soil
JF - Plant and Soil
SN - 0032-079X
ER -