Nitrogen but not phosphorus addition affects symbiotic N2 fixation by legumes in natural and semi-natural grasslands located on four continents

Eduardo Vázquez; Per Marten Schleuss; Elizabeth T. Borer; Miguel N. Bugalho; Maria C. Caldeira; Nico Eisenhauer; Anu Eskelinen; Philip A. Fay; Sylvia Haider; Anke Jentsch; Kevin P. Kirkman; Rebecca L. McCulley; Pablo L. Peri; Jodi Price; Anna E. Richards; Anita C. Risch; Christiane Roscher; Martin Schütz; Eric W. Seabloom; Rachel J. Standish; Carly J. Stevens; Michelle J. Tedder; Risto Virtanen; Marie Spohn

doi:10.1007/s11104-022-05498-y

Nitrogen but not phosphorus addition affects symbiotic N₂ fixation by legumes in natural and semi-natural grasslands located on four continents

Research output: Journal contributions › Journal articles › Research › peer-review

Standard

Nitrogen but not phosphorus addition affects symbiotic N₂ fixation by legumes in natural and semi-natural grasslands located on four continents. / Vázquez, Eduardo; Schleuss, Per Marten; Borer, Elizabeth T. et al.
In: Plant and Soil, Vol. 478, No. 1-2, 09.2022, p. 689-707.

Research output: Journal contributions › Journal articles › Research › peer-review

Harvard

Vázquez, E, Schleuss, PM, Borer, ET, Bugalho, MN, Caldeira, MC, Eisenhauer, N, Eskelinen, A, Fay, PA, Haider, S, Jentsch, A, Kirkman, KP, McCulley, RL, Peri, PL, Price, J, Richards, AE, Risch, AC, Roscher, C, Schütz, M, Seabloom, EW, Standish, RJ, Stevens, CJ, Tedder, MJ, Virtanen, R & Spohn, M 2022, 'Nitrogen but not phosphorus addition affects symbiotic N₂ fixation by legumes in natural and semi-natural grasslands located on four continents', Plant and Soil, vol. 478, no. 1-2, pp. 689-707. https://doi.org/10.1007/s11104-022-05498-y

APA

Vázquez, E., Schleuss, P. M., Borer, E. T., Bugalho, M. N., Caldeira, M. C., Eisenhauer, N., Eskelinen, A., Fay, P. A., Haider, S., Jentsch, A., Kirkman, K. P., McCulley, R. L., Peri, P. L., Price, J., Richards, A. E., Risch, A. C., Roscher, C., Schütz, M., Seabloom, E. W., ... Spohn, M. (2022). Nitrogen but not phosphorus addition affects symbiotic N₂ fixation by legumes in natural and semi-natural grasslands located on four continents. Plant and Soil, 478(1-2), 689-707. https://doi.org/10.1007/s11104-022-05498-y

Vancouver

Vázquez E, Schleuss PM, Borer ET, Bugalho MN, Caldeira MC, Eisenhauer N et al. Nitrogen but not phosphorus addition affects symbiotic N₂ fixation by legumes in natural and semi-natural grasslands located on four continents. Plant and Soil. 2022 Sept;478(1-2):689-707. doi: 10.1007/s11104-022-05498-y

Bibtex

@article{6a7a4c53a0a444ceb9eb2522cdf66ca9,

title = "Nitrogen but not phosphorus addition affects symbiotic N2 fixation by legumes in natural and semi-natural grasslands located on four continents",

abstract = "Background and aims: The amount of nitrogen (N) derived from symbiotic N2 fixation by legumes in grasslands might be affected by anthropogenic N and phosphorus (P) inputs, but the underlying mechanisms are not known. Methods: We evaluated symbiotic N2 fixation in 17 natural and semi-natural grasslands on four continents that are subjected to the same full-factorial N and P addition experiment, using the 15N natural abundance method. Results: N as well as combined N and P (NP) addition reduced aboveground legume biomass by 65% and 45%, respectively, compared to the control, whereas P addition had no significant impact. Addition of N and/or P had no significant effect on the symbiotic N2 fixation per unit legume biomass. In consequence, the amount of N fixed annually per grassland area was less than half in the N addition treatments compared to control and P addition, irrespective of whether the dominant legumes were annuals or perennials. Conclusion: Our results reveal that N addition mainly impacts symbiotic N2 fixation via reduced biomass of legumes rather than changes in N2 fixation per unit legume biomass. The results show that soil N enrichment by anthropogenic activities significantly reduces N2 fixation in grasslands, and these effects cannot be reversed by additional P amendment.",

keywords = "Biology, Grasslands, Legumes, Nitrogen addition, Nutrient Network (NutNet), Phosphorus addition, N natural abundance method",

author = "Eduardo V{\'a}zquez and Schleuss, {Per Marten} and Borer, {Elizabeth T.} and Bugalho, {Miguel N.} and Caldeira, {Maria C.} and Nico Eisenhauer and Anu Eskelinen and Fay, {Philip A.} and Sylvia Haider and Anke Jentsch and Kirkman, {Kevin P.} and McCulley, {Rebecca L.} and Peri, {Pablo L.} and Jodi Price and Richards, {Anna E.} and Risch, {Anita C.} and Christiane Roscher and Martin Sch{\"u}tz and Seabloom, {Eric W.} and Standish, {Rachel J.} and Stevens, {Carly J.} and Tedder, {Michelle J.} and Risto Virtanen and Marie Spohn",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = sep,

doi = "10.1007/s11104-022-05498-y",

language = "English",

volume = "478",

pages = "689--707",

journal = "Plant and Soil",

issn = "0032-079X",

publisher = "Springer Science and Business Media Deutschland",

number = "1-2",

}

RIS

TY - JOUR

T1 - Nitrogen but not phosphorus addition affects symbiotic N2 fixation by legumes in natural and semi-natural grasslands located on four continents

AU - Vázquez, Eduardo

AU - Schleuss, Per Marten

AU - Borer, Elizabeth T.

AU - Bugalho, Miguel N.

AU - Caldeira, Maria C.

AU - Eisenhauer, Nico

AU - Eskelinen, Anu

AU - Fay, Philip A.

AU - Haider, Sylvia

AU - Jentsch, Anke

AU - Kirkman, Kevin P.

AU - McCulley, Rebecca L.

AU - Peri, Pablo L.

AU - Price, Jodi

AU - Richards, Anna E.

AU - Risch, Anita C.

AU - Roscher, Christiane

AU - Schütz, Martin

AU - Seabloom, Eric W.

AU - Standish, Rachel J.

AU - Stevens, Carly J.

AU - Tedder, Michelle J.

AU - Virtanen, Risto

AU - Spohn, Marie

PY - 2022/9

Y1 - 2022/9

N2 - Background and aims: The amount of nitrogen (N) derived from symbiotic N2 fixation by legumes in grasslands might be affected by anthropogenic N and phosphorus (P) inputs, but the underlying mechanisms are not known. Methods: We evaluated symbiotic N2 fixation in 17 natural and semi-natural grasslands on four continents that are subjected to the same full-factorial N and P addition experiment, using the 15N natural abundance method. Results: N as well as combined N and P (NP) addition reduced aboveground legume biomass by 65% and 45%, respectively, compared to the control, whereas P addition had no significant impact. Addition of N and/or P had no significant effect on the symbiotic N2 fixation per unit legume biomass. In consequence, the amount of N fixed annually per grassland area was less than half in the N addition treatments compared to control and P addition, irrespective of whether the dominant legumes were annuals or perennials. Conclusion: Our results reveal that N addition mainly impacts symbiotic N2 fixation via reduced biomass of legumes rather than changes in N2 fixation per unit legume biomass. The results show that soil N enrichment by anthropogenic activities significantly reduces N2 fixation in grasslands, and these effects cannot be reversed by additional P amendment.

AB - Background and aims: The amount of nitrogen (N) derived from symbiotic N2 fixation by legumes in grasslands might be affected by anthropogenic N and phosphorus (P) inputs, but the underlying mechanisms are not known. Methods: We evaluated symbiotic N2 fixation in 17 natural and semi-natural grasslands on four continents that are subjected to the same full-factorial N and P addition experiment, using the 15N natural abundance method. Results: N as well as combined N and P (NP) addition reduced aboveground legume biomass by 65% and 45%, respectively, compared to the control, whereas P addition had no significant impact. Addition of N and/or P had no significant effect on the symbiotic N2 fixation per unit legume biomass. In consequence, the amount of N fixed annually per grassland area was less than half in the N addition treatments compared to control and P addition, irrespective of whether the dominant legumes were annuals or perennials. Conclusion: Our results reveal that N addition mainly impacts symbiotic N2 fixation via reduced biomass of legumes rather than changes in N2 fixation per unit legume biomass. The results show that soil N enrichment by anthropogenic activities significantly reduces N2 fixation in grasslands, and these effects cannot be reversed by additional P amendment.

KW - Biology

KW - Grasslands

KW - Legumes

KW - Nitrogen addition

KW - Nutrient Network (NutNet)

KW - Phosphorus addition

KW - N natural abundance method

UR - http://www.scopus.com/inward/record.url?scp=85132281198&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/9d022839-d8db-3208-ab30-dd6e60927501/

U2 - 10.1007/s11104-022-05498-y

DO - 10.1007/s11104-022-05498-y

M3 - Journal articles

AN - SCOPUS:85132281198

VL - 478

SP - 689

EP - 707

JO - Plant and Soil

JF - Plant and Soil

SN - 0032-079X

IS - 1-2

ER -

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DOI

https://doi.org/10.1007/s11104-022-05498-y
Final published version

Nitrogen but not phosphorus addition affects symbiotic N₂ fixation by legumes in natural and semi-natural grasslands located on four continents

Standard

Harvard

APA

Vancouver

Bibtex

RIS

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