Measuring plant root traits under controlled and field conditions: Step-by-step procedures
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Measuring plant root traits under controlled and field conditions : Step-by-step procedures. / Delory, Benjamin; Weidlich, Emanuela W. A. ; van Duijnen, Richard et al.
Root Development: Methods and Protocols. ed. / Daniela Ristova; Elke Barbez. New York : Humana Press, 2018. p. 3-22 (Methods in Molecular Biology; Vol. 1761).Research output: Contributions to collected editions/works › Contributions to collected editions/anthologies › Research › peer-review
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TY - CHAP
T1 - Measuring plant root traits under controlled and field conditions
T2 - Step-by-step procedures
AU - Delory, Benjamin
AU - Weidlich, Emanuela W. A.
AU - van Duijnen, Richard
AU - Pagès, Loïc
AU - Temperton, Victoria Martine
PY - 2018
Y1 - 2018
N2 - In this chapter, we present methods that we routinely use to measure plant root traits in the field and under controlled environmental conditions (using rhizoboxes). We describe procedures to (1) collect, wash, and store root samples, (2) acquire images of washed root samples, and (3) measure root traits using image analysis. In addition, we also describe sampling methods for studying belowground productivity, soil exploration, and root distribution in the first soil layers at the community level (soil coring and ingrowth core method). Because the use of rhizoboxes allows a nondestructive and dynamic measurement of traits hardly accessible in the field, a section of this chapter is devoted to the acquisition and analysis of images of roots growing in rhizoboxes.
AB - In this chapter, we present methods that we routinely use to measure plant root traits in the field and under controlled environmental conditions (using rhizoboxes). We describe procedures to (1) collect, wash, and store root samples, (2) acquire images of washed root samples, and (3) measure root traits using image analysis. In addition, we also describe sampling methods for studying belowground productivity, soil exploration, and root distribution in the first soil layers at the community level (soil coring and ingrowth core method). Because the use of rhizoboxes allows a nondestructive and dynamic measurement of traits hardly accessible in the field, a section of this chapter is devoted to the acquisition and analysis of images of roots growing in rhizoboxes.
KW - Image analysis
KW - Ingrowth core method
KW - Phenotyping
KW - Rhizobox
KW - Root staining
KW - Root traits
KW - Root washing
KW - Soil coring
KW - Ecosystems Research
UR - http://www.scopus.com/inward/record.url?scp=85043793431&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7747-5_1
DO - 10.1007/978-1-4939-7747-5_1
M3 - Contributions to collected editions/anthologies
C2 - 29525945
AN - SCOPUS:85043793431
SN - 978-1-4939-7746-8
T3 - Methods in Molecular Biology
SP - 3
EP - 22
BT - Root Development
A2 - Ristova, Daniela
A2 - Barbez, Elke
PB - Humana Press
CY - New York
ER -