MALDI-TOF MS-Based Lipidomic Profile of Honey and Bee Pollen
Research output: Journal contributions › Journal articles › Research › peer-review
Standard
In: ACS Agricultural Science and Technology, Vol. 5, No. 12, 15.12.2025, p. 2585-2595.
Research output: Journal contributions › Journal articles › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - MALDI-TOF MS-Based Lipidomic Profile of Honey and Bee Pollen
AU - Jano, Ana
AU - Fuente-Ballesteros, Adrián
AU - Tapia, Jesús A.
AU - Valverde, Silvia
AU - Ares, Ana M.
AU - Bernal, José
N1 - Publisher Copyright: © 2025 The Authors. Published by American Chemical Society
PY - 2025/12/15
Y1 - 2025/12/15
N2 - The increasing demand for bee-derived products such as honey and bee pollen has led to a rise in adulteration and mislabeling, making it essential to develop reliable tools for authentication. Lipids, which are found in both matrices, are potential biomarkers for tracing their origin and may be used for detecting fraud. In this work, a solid–liquid extraction using hexane:isopropanol (10:1, v/v) followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was optimized. The method was applied for tentative lipid screening of 15 honeys and 13 bee pollens showing a total number of lipids above 700, including fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, and sterol lipids. For the first time, a principal component analysis was carried out for botanical and geographical origin, classifying most of the samples correctly. Additionally, the method was categorized as green (environmentally friendly) and blue (practical).
AB - The increasing demand for bee-derived products such as honey and bee pollen has led to a rise in adulteration and mislabeling, making it essential to develop reliable tools for authentication. Lipids, which are found in both matrices, are potential biomarkers for tracing their origin and may be used for detecting fraud. In this work, a solid–liquid extraction using hexane:isopropanol (10:1, v/v) followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was optimized. The method was applied for tentative lipid screening of 15 honeys and 13 bee pollens showing a total number of lipids above 700, including fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, and sterol lipids. For the first time, a principal component analysis was carried out for botanical and geographical origin, classifying most of the samples correctly. Additionally, the method was categorized as green (environmentally friendly) and blue (practical).
KW - bee product
KW - biomarker
KW - food authentication
KW - food chemistry
KW - green analytical chemistry
KW - lipid profiling
KW - lipidomics
KW - MALDI-TOF MS
KW - metrics
KW - PCA
KW - principal component analysis
KW - Chemistry
UR - https://www.scopus.com/pages/publications/105024857492
U2 - 10.1021/acsagscitech.5c00883
DO - 10.1021/acsagscitech.5c00883
M3 - Journal articles
AN - SCOPUS:105024857492
VL - 5
SP - 2585
EP - 2595
JO - ACS Agricultural Science and Technology
JF - ACS Agricultural Science and Technology
SN - 2692-1952
IS - 12
ER -