TY - JOUR

T1 - MALDI-TOF MS-Based Lipidomic Profile of Honey and Bee Pollen

AU - Jano, Ana

AU - Fuente-Ballesteros, Adrián

AU - Tapia, Jesús A.

AU - Valverde, Silvia

AU - Ares, Ana M.

AU - Bernal, José

N1 - Publisher Copyright: © 2025 The Authors. Published by American Chemical Society

PY - 2025/12/15

Y1 - 2025/12/15

N2 - The increasing demand for bee-derived products such as honey and bee pollen has led to a rise in adulteration and mislabeling, making it essential to develop reliable tools for authentication. Lipids, which are found in both matrices, are potential biomarkers for tracing their origin and may be used for detecting fraud. In this work, a solid–liquid extraction using hexane:isopropanol (10:1, v/v) followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was optimized. The method was applied for tentative lipid screening of 15 honeys and 13 bee pollens showing a total number of lipids above 700, including fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, and sterol lipids. For the first time, a principal component analysis was carried out for botanical and geographical origin, classifying most of the samples correctly. Additionally, the method was categorized as green (environmentally friendly) and blue (practical).

AB - The increasing demand for bee-derived products such as honey and bee pollen has led to a rise in adulteration and mislabeling, making it essential to develop reliable tools for authentication. Lipids, which are found in both matrices, are potential biomarkers for tracing their origin and may be used for detecting fraud. In this work, a solid–liquid extraction using hexane:isopropanol (10:1, v/v) followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was optimized. The method was applied for tentative lipid screening of 15 honeys and 13 bee pollens showing a total number of lipids above 700, including fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, and sterol lipids. For the first time, a principal component analysis was carried out for botanical and geographical origin, classifying most of the samples correctly. Additionally, the method was categorized as green (environmentally friendly) and blue (practical).

KW - bee product

KW - biomarker

KW - food authentication

KW - food chemistry

KW - green analytical chemistry

KW - lipid profiling

KW - lipidomics

KW - MALDI-TOF MS

KW - metrics

KW - PCA

KW - principal component analysis

KW - Chemistry

UR - https://www.scopus.com/pages/publications/105024857492

U2 - 10.1021/acsagscitech.5c00883

DO - 10.1021/acsagscitech.5c00883

M3 - Journal articles

AN - SCOPUS:105024857492

VL - 5

SP - 2585

EP - 2595

JO - ACS Agricultural Science and Technology

JF - ACS Agricultural Science and Technology

SN - 2692-1952

IS - 12

ER -