Intracellular Accumulation of Linezolid in Escherichia Coli, Citrobacter Freundii and Enterobacter Aerogenes: Role of Enhanced Efflux Pump Activity and Inactivation

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Intracellular Accumulation of Linezolid in Escherichia Coli, Citrobacter Freundii and Enterobacter Aerogenes: Role of Enhanced Efflux Pump Activity and Inactivation. / Schumacher, Anja; Trittler, Rainer; Bohnert, Jürgen A. et al.
In: Journal of Antimicrobial Chemotherapy, Vol. 59, No. 6, 06.2007, p. 1261-1264.

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@article{2a479fbdf8cc4f53af4499265dc8fe34,
title = "Intracellular Accumulation of Linezolid in Escherichia Coli, Citrobacter Freundii and Enterobacter Aerogenes: Role of Enhanced Efflux Pump Activity and Inactivation",
abstract = "Objectives: The oxazolidinone class of antibiotics such as linezolid have a narrow spectrum of activity that targets Gram-positive bacteria. We hypothesized that the poor activity of linezolid in Gram-negative bacteria is in part caused by relatively low intracellular concentration due to efflux. Methods: Using whole cell accumulation assays we estimated the intracellular concentration of linezolid in Escherichia coli and other Enterobacteriaceae. We included test strains with enhanced RND-type multidrug efflux pump activity and with genetic inactivation of the pump or functional inhibition by carbonyl cyanide m-chlorophenylhydrazone as inhibitor of the proton motive force or 1-(1-naphthylmethyl)-piperazine (NMP), an efflux pump inhibitor. Results: Consistent with susceptibility studies, enhanced pump activity caused decreased accumulation, and pump inactivation and inhibition caused increased accumulation, of linezolid. The accumulation levels in test strains of E. coli, Citrobacter freundii and Enterobacter aerogenes with functional pumps were lower than in control strains of Staphylococcus aureus and Enterococcus faecium, but were higher after pump inactivation and correlated with ethidium bromide and pyronin Y accumulation. Conclusions: The intracellular concentration of linezolidis comparatively low owing to efficient efflux of the drug and could be increased substantially by inhibition of RND-type efflux pumps. {\textcopyright} The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.",
keywords = "efflux pumps, Enterobacteriaceae, multidrug resistance, oxazolidinones, Chemistry",
author = "Anja Schumacher and Rainer Trittler and Bohnert, {J{\"u}rgen A.} and Klaus K{\"u}mmerer and Jean-Marie Pages and Kern, {Winfried V.}",
note = "Funding Information: This study was supported in part by the Landesstiftung Baden-W{\"u}rttemberg. Pfizer provided a grant for HPLC measurements of linezolid.",
year = "2007",
month = jun,
doi = "10.1093/jac/dkl380",
language = "English",
volume = "59",
pages = "1261--1264",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "6",

}

RIS

TY - JOUR

T1 - Intracellular Accumulation of Linezolid in Escherichia Coli, Citrobacter Freundii and Enterobacter Aerogenes

T2 - Role of Enhanced Efflux Pump Activity and Inactivation

AU - Schumacher, Anja

AU - Trittler, Rainer

AU - Bohnert, Jürgen A.

AU - Kümmerer, Klaus

AU - Pages, Jean-Marie

AU - Kern, Winfried V.

N1 - Funding Information: This study was supported in part by the Landesstiftung Baden-Württemberg. Pfizer provided a grant for HPLC measurements of linezolid.

PY - 2007/6

Y1 - 2007/6

N2 - Objectives: The oxazolidinone class of antibiotics such as linezolid have a narrow spectrum of activity that targets Gram-positive bacteria. We hypothesized that the poor activity of linezolid in Gram-negative bacteria is in part caused by relatively low intracellular concentration due to efflux. Methods: Using whole cell accumulation assays we estimated the intracellular concentration of linezolid in Escherichia coli and other Enterobacteriaceae. We included test strains with enhanced RND-type multidrug efflux pump activity and with genetic inactivation of the pump or functional inhibition by carbonyl cyanide m-chlorophenylhydrazone as inhibitor of the proton motive force or 1-(1-naphthylmethyl)-piperazine (NMP), an efflux pump inhibitor. Results: Consistent with susceptibility studies, enhanced pump activity caused decreased accumulation, and pump inactivation and inhibition caused increased accumulation, of linezolid. The accumulation levels in test strains of E. coli, Citrobacter freundii and Enterobacter aerogenes with functional pumps were lower than in control strains of Staphylococcus aureus and Enterococcus faecium, but were higher after pump inactivation and correlated with ethidium bromide and pyronin Y accumulation. Conclusions: The intracellular concentration of linezolidis comparatively low owing to efficient efflux of the drug and could be increased substantially by inhibition of RND-type efflux pumps. © The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.

AB - Objectives: The oxazolidinone class of antibiotics such as linezolid have a narrow spectrum of activity that targets Gram-positive bacteria. We hypothesized that the poor activity of linezolid in Gram-negative bacteria is in part caused by relatively low intracellular concentration due to efflux. Methods: Using whole cell accumulation assays we estimated the intracellular concentration of linezolid in Escherichia coli and other Enterobacteriaceae. We included test strains with enhanced RND-type multidrug efflux pump activity and with genetic inactivation of the pump or functional inhibition by carbonyl cyanide m-chlorophenylhydrazone as inhibitor of the proton motive force or 1-(1-naphthylmethyl)-piperazine (NMP), an efflux pump inhibitor. Results: Consistent with susceptibility studies, enhanced pump activity caused decreased accumulation, and pump inactivation and inhibition caused increased accumulation, of linezolid. The accumulation levels in test strains of E. coli, Citrobacter freundii and Enterobacter aerogenes with functional pumps were lower than in control strains of Staphylococcus aureus and Enterococcus faecium, but were higher after pump inactivation and correlated with ethidium bromide and pyronin Y accumulation. Conclusions: The intracellular concentration of linezolidis comparatively low owing to efficient efflux of the drug and could be increased substantially by inhibition of RND-type efflux pumps. © The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.

KW - efflux pumps

KW - Enterobacteriaceae

KW - multidrug resistance

KW - oxazolidinones

KW - Chemistry

UR - http://www.scopus.com/inward/record.url?scp=34447553357&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/96cb3c38-fdf6-36e2-a25a-6a2292502df4/

U2 - 10.1093/jac/dkl380

DO - 10.1093/jac/dkl380

M3 - Journal articles

C2 - 16971414

VL - 59

SP - 1261

EP - 1264

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 6

ER -

DOI