TY - JOUR

T1 - Interference of magnesium corrosion with tetrazolium-based cytotoxicity assays

AU - Fischer, Janine

AU - Prosenc, Marc H.

AU - Wolff, Martin

AU - Hort, Norbert

AU - Willumeit, Regine

AU - Feyerabend, Frank

PY - 2010/5

Y1 - 2010/5

N2 - Magnesium (Mg) alloys are promising materials for the development of biodegradable implants. However, the current in vitro test procedures for cytotoxicity, cell viability and proliferation are not always suitable for this class of materials. In this paper we show that tetrazolium-salt-based assays, which are widely used in practice, are influenced by the corrosion products of Mg-based alloys. Corroded Mg converts tetrazolium salts to formazan, leading to a higher background and falsifying the results of cell viability. Tetrazolium-based assays are therefore not a useful tool for testing the cytotoxicity of Mg in static in vitro assays.

AB - Magnesium (Mg) alloys are promising materials for the development of biodegradable implants. However, the current in vitro test procedures for cytotoxicity, cell viability and proliferation are not always suitable for this class of materials. In this paper we show that tetrazolium-salt-based assays, which are widely used in practice, are influenced by the corrosion products of Mg-based alloys. Corroded Mg converts tetrazolium salts to formazan, leading to a higher background and falsifying the results of cell viability. Tetrazolium-based assays are therefore not a useful tool for testing the cytotoxicity of Mg in static in vitro assays.

KW - Cytotoxicity

KW - Magnesium

KW - MTT

KW - Tetrazolium-based assays

KW - XTT

KW - Engineering

UR - http://www.scopus.com/inward/record.url?scp=77953082897&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2009.10.020

DO - 10.1016/j.actbio.2009.10.020

M3 - Journal articles

C2 - 19837191

AN - SCOPUS:77953082897

VL - 6

SP - 1813

EP - 1823

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 5

ER -