Identification of Dermatophyte and mold species by MALDI-TOF Mass Spectrometry

Research output: Journal contributionsConference abstract in journalResearchpeer-review

Authors

  • Marco Reich
  • Maik Stark
  • Gert Huesgen
  • Philipp P. Bosshard
  • Stefan Borgmann
Routine procedures for dermatophyte and mold identification rely on an
examination of strain characteristics and microscopic morphology.
Conventional methods used to identify dermatophytes and molds are often
time-consuming and may be inconclusive because of atypical microscopic or
colony morphology. The present study evaluates a promising alternative based
on the analysis of clinical fungal isolates by MALDI-TOF mass spectrometry.
A total of 63 isolates belonging to 27 species of dermatophytes and molds were
analyzed. The isolates were obtained from reference samples
(„Ringversuchsproben“, INSTAND e.V., n= 17) as well as from reliable
identified clinical isolates from a variety of specimens including skin, nail
scrapings and hair fragments. The clinical isolates were obtained from the
University Hospital of Zurich (Department of Dermatology, n= 30) and from
the Synlab Laboratory Leinfelden (n=16). Because many fungi attached very
strong to agar, sample harvesting without agar contamination was not possible.
Therefore, the isolates were additionally inoculated in liquid culture media.
Almost 93% (59 of 63) of the MALDI-TOF identification results matched to
those of conventional methods respectively to the identity of the reference
samples. Four mismatches were obtained from clinical isolates. Trichophyton
mentagropytes var. Interdigitale was identified incorrectly as Trichophyton
tonsurans (n= 4). However these mismatches arise from database discrepancy
and could be eliminated by further development of the database.In Summary,
MALDI- TOF analyses of common dermatophytes and molds resulted in
reliable species identification. Furthermore, this procedure allowed very rapid
identification results. The isolates could be identified already after a growth
time of 4-14 days, when inoculated in liquid culture media.
Original languageEnglish
Article numberEKP09
JournalInternational Journal of Medical Microbiology
Volume299
Issue numberSupplement 1
Pages (from-to)13
Number of pages1
ISSN1438-4221
DOIs
Publication statusPublished - 01.09.2009
Externally publishedYes

    Research areas

  • Chemistry
  • Biology
  • Animals, Bacterial Physiological Phenomena, Cell Physiological Phenomena, Cells/microbiology, Insecta/cytology, Symbiosis

Recently viewed

Publications

  1. Der BilWiss-2.0-Test
  2. “€14,875?!”
  3. Schreibwissenschaft methodisch
  4. Bat pest control contributes to food security in Thailand
  5. Subject Citizen. The Ambiguity of Citizenship and its Colonial Laboratories
  6. Germany must go back to its low-carbon future
  7. Tag questions across Irish English and British English
  8. Localized accelerated degradation of magnesium
  9. Vorwort
  10. Fehler beim Controlling
  11. Der Übersetzer als Performer
  12. ‚Ich sehe was, was Du nicht siehst‘
  13. The safe-and-sustainable-by-design concept
  14. NAVIGATING PROFESSIONAL CAREERS AND INTERNAL ACTIVISM
  15. Briefe schreiben in Klasse 9 und 11
  16. Kostenmanagement
  17. The Influence of Personality on Career Decisiveness of Business Students
  18. Fishing in the Amazonian Forest
  19. Eucamaragnathus desenderi, a new ground beetle species from Africa (Coleoptera, Carabidae)
  20. BAuA-Working Time Survey (BAuA-WTS)
  21. Green in grey
  22. BRANGE EFFECTS IN HEDONIC EVALUATION OF OLFACTORY STIMULI
  23. Art. 17 OECD MA
  24. The Need for Healthy Leadership in the Health Care Sector
  25. Gefühle, Werte und das ausgedehnte Selbst
  26. Maximum strength and power as determinants of on-ice sprint performance in elite U16 to adult ice hockey players
  27. 'Freiwurf Hamburg': Qualitative Evaluation of an Inclusive Sports Project
  28. Analytical prediction of roughness after ball burnishing of thermally coated surfaces
  29. The role of training in implementing corporate sustainability
  30. Lee Daniels' The Butler
  31. CO2 taxation and competitiveness of the German economy - an analysis with the macroeconomic information system (MIS)
  32. Adaptation of the Brine Shrimp Artemia Salina (Branchiopoda: Anostraca) to Filter-Feeding
  33. Depicting Women in Brazilian Social Realism: A Transnational and Computational Analysis
  34. Produce, publish, share - A YouTube video contest
  35. The Science and Practice of Landscape Stewardship, Claudia Bieling, Tobias Plieninger. Cambridge University Press (2017)
  36. Die Sintflut
  37. Kant meets Minkowski