Fatty Acid Profile of the Initial Oral Biofilm (Pellicle): an In-Situ Study

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Fatty Acid Profile of the Initial Oral Biofilm (Pellicle): an In-Situ Study. / Reich, M.; Kümmerer, K.; Al-Ahmad, A. et al.
In: Lipids, Vol. 48, No. 9, 09.2013, p. 929-937.

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Reich M, Kümmerer K, Al-Ahmad A, Hannig C. Fatty Acid Profile of the Initial Oral Biofilm (Pellicle): an In-Situ Study. Lipids. 2013 Sept;48(9):929-937. doi: 10.1007/s11745-013-3822-2

Bibtex

@article{a262d636e3ee47508561673dcc580ccb,
title = "Fatty Acid Profile of the Initial Oral Biofilm (Pellicle): an In-Situ Study",
abstract = "The first step of bioadhesion on dental surfaces is the formation of the acquired pellicle. This mainly acellular layer is formed instantaneously on all solid surfaces exposed to oral fluids. It is composed of proteins, glycoproteins and lipids. However, information on the lipid composition is sparse. The aim of the present study was to evaluate the fatty acid (FA) profile of the in-situ pellicle for the first time. Furthermore, the impact of rinses with safflower oil on the pellicle's FA composition was investigated. Pellicles were formed in situ on bovine enamel slabs mounted on individual upper jaw splints. The splints were carried by ten subjects over durations of 3-240 min. After comprehensive sample preparation, gas chromatography coupled with electron impact ionization mass spectrometry (GC-EI/MS) was used in order to characterize qualitatively and quantitatively a wide range of FA (C 12-C 24). The relative FA profiles of the pellicle samples gained from different subjects were remarkably similar, whereas the amount of FA showed significant interindividual variability. An increase in FA in the pellicle was observed over time. The application of rinses with safflower oil resulted in an accumulation of its specific FA in the pellicle. Pellicle formation is a highly selective process that does not correlate directly with salivary composition, as shown for FA.",
keywords = "Chemistry, Bioadhesion, Biofilm, Extraction, Fatty acid composition, GC-MS, In situ, Pellicle, Safflower oil, Saliva, Transesterification, Sustainability Science",
author = "M. Reich and K. K{\"u}mmerer and A. Al-Ahmad and C. Hannig",
year = "2013",
month = sep,
doi = "10.1007/s11745-013-3822-2",
language = "English",
volume = "48",
pages = "929--937",
journal = "Lipids",
issn = "0024-4201",
publisher = "Springer",
number = "9",

}

RIS

TY - JOUR

T1 - Fatty Acid Profile of the Initial Oral Biofilm (Pellicle)

T2 - an In-Situ Study

AU - Reich, M.

AU - Kümmerer, K.

AU - Al-Ahmad, A.

AU - Hannig, C.

PY - 2013/9

Y1 - 2013/9

N2 - The first step of bioadhesion on dental surfaces is the formation of the acquired pellicle. This mainly acellular layer is formed instantaneously on all solid surfaces exposed to oral fluids. It is composed of proteins, glycoproteins and lipids. However, information on the lipid composition is sparse. The aim of the present study was to evaluate the fatty acid (FA) profile of the in-situ pellicle for the first time. Furthermore, the impact of rinses with safflower oil on the pellicle's FA composition was investigated. Pellicles were formed in situ on bovine enamel slabs mounted on individual upper jaw splints. The splints were carried by ten subjects over durations of 3-240 min. After comprehensive sample preparation, gas chromatography coupled with electron impact ionization mass spectrometry (GC-EI/MS) was used in order to characterize qualitatively and quantitatively a wide range of FA (C 12-C 24). The relative FA profiles of the pellicle samples gained from different subjects were remarkably similar, whereas the amount of FA showed significant interindividual variability. An increase in FA in the pellicle was observed over time. The application of rinses with safflower oil resulted in an accumulation of its specific FA in the pellicle. Pellicle formation is a highly selective process that does not correlate directly with salivary composition, as shown for FA.

AB - The first step of bioadhesion on dental surfaces is the formation of the acquired pellicle. This mainly acellular layer is formed instantaneously on all solid surfaces exposed to oral fluids. It is composed of proteins, glycoproteins and lipids. However, information on the lipid composition is sparse. The aim of the present study was to evaluate the fatty acid (FA) profile of the in-situ pellicle for the first time. Furthermore, the impact of rinses with safflower oil on the pellicle's FA composition was investigated. Pellicles were formed in situ on bovine enamel slabs mounted on individual upper jaw splints. The splints were carried by ten subjects over durations of 3-240 min. After comprehensive sample preparation, gas chromatography coupled with electron impact ionization mass spectrometry (GC-EI/MS) was used in order to characterize qualitatively and quantitatively a wide range of FA (C 12-C 24). The relative FA profiles of the pellicle samples gained from different subjects were remarkably similar, whereas the amount of FA showed significant interindividual variability. An increase in FA in the pellicle was observed over time. The application of rinses with safflower oil resulted in an accumulation of its specific FA in the pellicle. Pellicle formation is a highly selective process that does not correlate directly with salivary composition, as shown for FA.

KW - Chemistry

KW - Bioadhesion

KW - Biofilm

KW - Extraction

KW - Fatty acid composition

KW - GC-MS

KW - In situ

KW - Pellicle

KW - Safflower oil

KW - Saliva

KW - Transesterification

KW - Sustainability Science

UR - http://www.scopus.com/inward/record.url?scp=84883185610&partnerID=8YFLogxK

U2 - 10.1007/s11745-013-3822-2

DO - 10.1007/s11745-013-3822-2

M3 - Journal articles

C2 - 23912324

VL - 48

SP - 929

EP - 937

JO - Lipids

JF - Lipids

SN - 0024-4201

IS - 9

ER -