Fatty Acid Profile of the Initial Oral Biofilm (Pellicle): an In-Situ Study
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Fatty Acid Profile of the Initial Oral Biofilm (Pellicle) : an In-Situ Study. / Reich, M.; Kümmerer, K.; Al-Ahmad, A. et al.
In: Lipids, Vol. 48, No. 9, 09.2013, p. 929-937.Research output: Journal contributions › Journal articles › Research › peer-review
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TY - JOUR
T1 - Fatty Acid Profile of the Initial Oral Biofilm (Pellicle)
T2 - an In-Situ Study
AU - Reich, M.
AU - Kümmerer, K.
AU - Al-Ahmad, A.
AU - Hannig, C.
PY - 2013/9
Y1 - 2013/9
N2 - The first step of bioadhesion on dental surfaces is the formation of the acquired pellicle. This mainly acellular layer is formed instantaneously on all solid surfaces exposed to oral fluids. It is composed of proteins, glycoproteins and lipids. However, information on the lipid composition is sparse. The aim of the present study was to evaluate the fatty acid (FA) profile of the in-situ pellicle for the first time. Furthermore, the impact of rinses with safflower oil on the pellicle's FA composition was investigated. Pellicles were formed in situ on bovine enamel slabs mounted on individual upper jaw splints. The splints were carried by ten subjects over durations of 3-240 min. After comprehensive sample preparation, gas chromatography coupled with electron impact ionization mass spectrometry (GC-EI/MS) was used in order to characterize qualitatively and quantitatively a wide range of FA (C 12-C 24). The relative FA profiles of the pellicle samples gained from different subjects were remarkably similar, whereas the amount of FA showed significant interindividual variability. An increase in FA in the pellicle was observed over time. The application of rinses with safflower oil resulted in an accumulation of its specific FA in the pellicle. Pellicle formation is a highly selective process that does not correlate directly with salivary composition, as shown for FA.
AB - The first step of bioadhesion on dental surfaces is the formation of the acquired pellicle. This mainly acellular layer is formed instantaneously on all solid surfaces exposed to oral fluids. It is composed of proteins, glycoproteins and lipids. However, information on the lipid composition is sparse. The aim of the present study was to evaluate the fatty acid (FA) profile of the in-situ pellicle for the first time. Furthermore, the impact of rinses with safflower oil on the pellicle's FA composition was investigated. Pellicles were formed in situ on bovine enamel slabs mounted on individual upper jaw splints. The splints were carried by ten subjects over durations of 3-240 min. After comprehensive sample preparation, gas chromatography coupled with electron impact ionization mass spectrometry (GC-EI/MS) was used in order to characterize qualitatively and quantitatively a wide range of FA (C 12-C 24). The relative FA profiles of the pellicle samples gained from different subjects were remarkably similar, whereas the amount of FA showed significant interindividual variability. An increase in FA in the pellicle was observed over time. The application of rinses with safflower oil resulted in an accumulation of its specific FA in the pellicle. Pellicle formation is a highly selective process that does not correlate directly with salivary composition, as shown for FA.
KW - Chemistry
KW - Bioadhesion
KW - Biofilm
KW - Extraction
KW - Fatty acid composition
KW - GC-MS
KW - In situ
KW - Pellicle
KW - Safflower oil
KW - Saliva
KW - Transesterification
KW - Sustainability Science
UR - http://www.scopus.com/inward/record.url?scp=84883185610&partnerID=8YFLogxK
U2 - 10.1007/s11745-013-3822-2
DO - 10.1007/s11745-013-3822-2
M3 - Journal articles
C2 - 23912324
VL - 48
SP - 929
EP - 937
JO - Lipids
JF - Lipids
SN - 0024-4201
IS - 9
ER -