1. //
  2. Start
  3. //
  4. Publications
  5. An Advanced Double Column-Switching Tech...
  6. Output formats
  7. //

An Advanced Double Column-Switching Technique (LC-LC) for Liquid Chromatography/Electrospray Ionisation Tandem Mass Spectrometry for Fully Automated Analysis of Caspofungin

Research output: Journal contributions › Journal articles › Research › peer-review

Standard

An Advanced Double Column-Switching Technique (LC-LC) for Liquid Chromatography/Electrospray Ionisation Tandem Mass Spectrometry for Fully Automated Analysis of Caspofungin. / Egle, Hannes; Trittler, Rainer; Kümmerer, Klaus.
In: Rapid Communications in Mass Spectrometry , Vol. 18, No. 23, 01.11.2004, p. 2871-2877.

Research output: Journal contributions › Journal articles › Research › peer-review

Harvard

Egle, H, Trittler, R & Kümmerer, K 2004, 'An Advanced Double Column-Switching Technique (LC-LC) for Liquid Chromatography/Electrospray Ionisation Tandem Mass Spectrometry for Fully Automated Analysis of Caspofungin', Rapid Communications in Mass Spectrometry , vol. 18, no. 23, pp. 2871-2877. https://doi.org/10.1002/rcm.1691

APA

Egle, H., Trittler, R., & Kümmerer, K. (2004). An Advanced Double Column-Switching Technique (LC-LC) for Liquid Chromatography/Electrospray Ionisation Tandem Mass Spectrometry for Fully Automated Analysis of Caspofungin. Rapid Communications in Mass Spectrometry , 18(23), 2871-2877. https://doi.org/10.1002/rcm.1691

Vancouver

Egle H, Trittler R, Kümmerer K. An Advanced Double Column-Switching Technique (LC-LC) for Liquid Chromatography/Electrospray Ionisation Tandem Mass Spectrometry for Fully Automated Analysis of Caspofungin. Rapid Communications in Mass Spectrometry . 2004 Nov 1;18(23):2871-2877. doi: 10.1002/rcm.1691

Bibtex

@article{d286d40083ce410fad8437b54949f7d9,
title = "An Advanced Double Column-Switching Technique (LC-LC) for Liquid Chromatography/Electrospray Ionisation Tandem Mass Spectrometry for Fully Automated Analysis of Caspofungin",
abstract = "Caspofungin (MK-0991; L-743,872) is the first representative of a new important class of antifungal agents, the glucan synthesis inhibitors. To the authors' best knowledge, to date only one high-performance liquid chromatography (HPLC) method has been published for the determination of caspofungin in serum. Severe difficulties with sorption were described. We developed a new method which addresses these difficulties using an advanced column-switching technique for fully automated analysis of caspofungin in serum without any pre-treatment. Extraction was performed automatically inline, using a diol column, followed by chromatography on a CN column. Detection was performed by electrospray ionisation tandem mass spectrometry (ESI-MS/MS) with isolation and fragmentation in the positive ion mode. Total analysis time was 30 min. Detection of caspofungin was achieved by retention time, isolation and fragmentation of the double positively charged caspofungin ion. This LC/MS assay was validated for between-run accuracy (max. 110%) and precision (max. CV 16.1%). The lower limit of quantification was 0.2 μg/mL. The analytical method with fully automated inline extraction of caspofungin described here removes the need for difficult and time-consuming sample pre-treatment. Sorption of caspofungin is not of importance. Additional advantages of the new method are that only a small quantity of serum (5 μL) is needed and that the method is very specific. Copyright {\textcopyright} 2004 John Wiley & Sons, Ltd.",
keywords = "accuracy, AGENTS, ANALYSIS, antifungal, ANTIFUNGAL AGENT, ASSAY, Caspofungin, chromatography, COLUMN, column switching, column-switching, detection, determination, Electrospray, EXTRACTION, GERMANY, HIGH PERFORMANCE LIQUID CHROMATOGRAPHY, HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY, HPLC, human plasma, INHIBITOR, INHIBITORS, Ionisation, KNOWLEDGE, LC, MS, liquid, liquid chromatography, LIQUID-CHROMATOGRAPHY, mass spectrometry, MASS-SPECTROMETRY, Method, MK-0991, MODE, pharmacokinetics, PRETREATMENT, QUANTIFICATION, RABBITS, retention, Serum, Sorption, SPECTROMETRY, tandem mass spectrometry, URINE, Chemistry",
author = "Hannes Egle and Rainer Trittler and Klaus K{\"u}mmerer",
note = "2004 John Wiley & Sons, Ltd.",
year = "2004",
month = nov,
day = "1",
doi = "10.1002/rcm.1691",
language = "English",
volume = "18",
pages = "2871--2877",
journal = "Rapid Communications in Mass Spectrometry ",
issn = "1097-0231",
publisher = "John Wiley & Sons Ltd.",
number = "23",

}

RIS

TY - JOUR

T1 - An Advanced Double Column-Switching Technique (LC-LC) for Liquid Chromatography/Electrospray Ionisation Tandem Mass Spectrometry for Fully Automated Analysis of Caspofungin

AU - Egle, Hannes

AU - Trittler, Rainer

AU - Kümmerer, Klaus

N1 - 2004 John Wiley & Sons, Ltd.

PY - 2004/11/1

Y1 - 2004/11/1

N2 - Caspofungin (MK-0991; L-743,872) is the first representative of a new important class of antifungal agents, the glucan synthesis inhibitors. To the authors' best knowledge, to date only one high-performance liquid chromatography (HPLC) method has been published for the determination of caspofungin in serum. Severe difficulties with sorption were described. We developed a new method which addresses these difficulties using an advanced column-switching technique for fully automated analysis of caspofungin in serum without any pre-treatment. Extraction was performed automatically inline, using a diol column, followed by chromatography on a CN column. Detection was performed by electrospray ionisation tandem mass spectrometry (ESI-MS/MS) with isolation and fragmentation in the positive ion mode. Total analysis time was 30 min. Detection of caspofungin was achieved by retention time, isolation and fragmentation of the double positively charged caspofungin ion. This LC/MS assay was validated for between-run accuracy (max. 110%) and precision (max. CV 16.1%). The lower limit of quantification was 0.2 μg/mL. The analytical method with fully automated inline extraction of caspofungin described here removes the need for difficult and time-consuming sample pre-treatment. Sorption of caspofungin is not of importance. Additional advantages of the new method are that only a small quantity of serum (5 μL) is needed and that the method is very specific. Copyright © 2004 John Wiley & Sons, Ltd.

AB - Caspofungin (MK-0991; L-743,872) is the first representative of a new important class of antifungal agents, the glucan synthesis inhibitors. To the authors' best knowledge, to date only one high-performance liquid chromatography (HPLC) method has been published for the determination of caspofungin in serum. Severe difficulties with sorption were described. We developed a new method which addresses these difficulties using an advanced column-switching technique for fully automated analysis of caspofungin in serum without any pre-treatment. Extraction was performed automatically inline, using a diol column, followed by chromatography on a CN column. Detection was performed by electrospray ionisation tandem mass spectrometry (ESI-MS/MS) with isolation and fragmentation in the positive ion mode. Total analysis time was 30 min. Detection of caspofungin was achieved by retention time, isolation and fragmentation of the double positively charged caspofungin ion. This LC/MS assay was validated for between-run accuracy (max. 110%) and precision (max. CV 16.1%). The lower limit of quantification was 0.2 μg/mL. The analytical method with fully automated inline extraction of caspofungin described here removes the need for difficult and time-consuming sample pre-treatment. Sorption of caspofungin is not of importance. Additional advantages of the new method are that only a small quantity of serum (5 μL) is needed and that the method is very specific. Copyright © 2004 John Wiley & Sons, Ltd.

KW - accuracy

KW - AGENTS

KW - ANALYSIS

KW - antifungal

KW - ANTIFUNGAL AGENT

KW - ASSAY

KW - Caspofungin

KW - chromatography

KW - COLUMN

KW - column switching

KW - column-switching

KW - detection

KW - determination

KW - Electrospray

KW - EXTRACTION

KW - GERMANY

KW - HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

KW - HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

KW - HPLC

KW - human plasma

KW - INHIBITOR

KW - INHIBITORS

KW - Ionisation

KW - KNOWLEDGE

KW - LC

KW - MS

KW - liquid

KW - liquid chromatography

KW - LIQUID-CHROMATOGRAPHY

KW - mass spectrometry

KW - MASS-SPECTROMETRY

KW - Method

KW - MK-0991

KW - MODE

KW - pharmacokinetics

KW - PRETREATMENT

KW - QUANTIFICATION

KW - RABBITS

KW - retention

KW - Serum

KW - Sorption

KW - SPECTROMETRY

KW - tandem mass spectrometry

KW - URINE

KW - Chemistry

UR - http://www.scopus.com/inward/record.url?scp=9644266735&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/7166b00d-a772-3009-bb61-a1f761b5658b/

U2 - 10.1002/rcm.1691

DO - 10.1002/rcm.1691

M3 - Journal articles

C2 - 15517540

VL - 18

SP - 2871

EP - 2877

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

SN - 1097-0231

IS - 23

ER -

Related by journal

Combination of different liquid chromatography/mass spectrometry technologies for the identification of transformation products of rhodamine B in groundwater

Mueller, A., Weiss, S. C., Seitz, W., Albert, R., Ruck, W. K. L., Weber, W. & Schulz, W., 15.03.2010, In: Rapid Comunications in Mass Spectrometry. 24, 5, p. 659-666 8 p.

Research output: Journal contributions › Journal articles › Research › peer-review

Other publications by the same author(s)

Accelerating the industrial transition with safe-and-sustainable-by-design (SSbD)

Soeteman-Hernández, L. G., Tickner, J. A., Dierckx, A., Kümmerer, K., Apel, C. & Strömberg, E., 17.02.2025, In: RSC Sustainability. 3, 5, p. 2185-2191 7 p.

Research output: Journal contributions › Scientific review articles › Research

Eco-pharma dilemma: Navigating environmental sustainability trade-offs within the lifecycle of pharmaceuticals – A comment

Moermond, C. T. A., Puhlmann, N., Pieters, L., Matharu, A., Boone, L., Dobbelaere, M., Proquin, H., Kümmerer, K., Ragas, A. M. J., Vidaurre, R., Venhuis, B. & De Smedt, D., 01.02.2025, In: Sustainable Chemistry and Pharmacy. 43, 10 p., 101893.

Research output: Journal contributions › Journal articles › Research › peer-review

Green chemistry and sustainable chemistry related to water challenges: Solutions and prospects in a changing climate

Mahjoub, B., Fersi, C., Bouteffeha, M. & Kümmerer, K., 01.04.2025, In: Current Opinion in Green and Sustainable Chemistry. 52, 11 p., 101000.

Research output: Journal contributions › Scientific review articles › Research

Inventing a secure future: material stewardship as chemistry's mission for sustainability

Matlin, S. A., Cornell, S. E., Kümmerer, K., Mahaffy, P. G. & Mehta, G., 14.01.2025, In: RSC Sustainability. 3, 2, p. 804-821 18 p., d4su00576g.

Research output: Journal contributions › Scientific review articles › Research

The complex spatio-temporal dynamics of organic micropollutants in tidal rivers

Schneider, J. R. L., Kümmerer, K., Logemann, J. & Radke, M., 01.03.2025, In: Chemosphere. 373, 10 p., 144180.

Research output: Journal contributions › Journal articles › Research › peer-review

DOI

Recently viewed

Activities

  1. IV. European Workshop on Clinical Reasoning and Decision Making - 2011
  2. Mentor for Lukas Vogelgsang
  3. Fakultät Management und Technologie (Organisation)
  4. Mentor for Hana Fehrenbach
  5. University of Gdansk, Polen (Externe Organisation)
  6. Power poles and tall trees adjacent to sunflower fields increase pest damage caused by the invasive Rose-ringed Parakeet in Israel
  7. 2nd International Colloquium on Adaption Intelligence of Factories 2018
  8. Les analyses de Leo Löwenthal sur la “conscience bourgeoise” dans la littérature
  9. QUA-LiS NRW. Modellvorhaben für die Entwicklung eines Blended-Learning-Formates
  10. What is Scientific Research? Critical reflections on criteria of transdisciplinary Sustainability Science
  11. Forschungsseminar, Universität zu Köln (CMR)
  12. Organizational Socialization: How can Organizations Support Refugee Newcomers’ Adjustment?
  13. College (Organisation)
  14. Insect Chemical Ecology 2014 - Doctoral Course - ICE 2014

Publications

  1. Article 32 Date of Application
  2. Defining value in sustainable business models
  3. Goffman’s Return to Las Vegas
  4. Erfolgreiche Business Intelligence-Projekte
  5. Cognition in High-Frequency Trading
  6. Effect of extrusion and rotary swaging on the microstructural evolution and properties of Mg-5Li-5.3Al-0.7Si alloy
  7. In search of the knowledge triangle for regional sustainable development: the role of universities
  8. Guest Editors of Special Issue:
  9. Can Becoming a Leader Change Your Personality?
  10. Comparison of wood volume estimates of young trees from terrestrial laser scan data
  11. Chemistry of POPs in the Atmosphere
  12. Integrating food security and biodiversity governance
  13. Participation in protected area governance
  14. Examining long-term impacts of a training programme to improve quality of IEP goals
  15. Transformation in a changing climate
  16. LivingCare - An autonomously learning, human centered home automation system
  17. Comparison of PSA-specific CD8+ CTL responses and antitumor immunity generated by plasmid DNA vaccines encoding PSA-HSP chimeric proteins
  18. Catch, bycatch and discards of the GalapagosMarine Reserve small-scale handline fishery
  19. Conclusion
  20. Catholics, Protestants and Muslims
  21. Mobile technologies, from telecommunication to media, ed. by Gerard Goggin ...
  22. Entrepreneuring as Multispecies Composting
  23. Crowdfunding
  24. Decoding media images of political leaders
  25. Shape-shifting
  26. Forschungsprojekt "Das Technische Bild"
  27. Architektur Campus Leuphana
  28. Sustainability-Oriented Innovation
  29. Connecting Some Dots
  30. The WTO’s Crisis
  31. The Identity/Policy Nexus in European Foreign Policy
  32. Picture-languages