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Identification of an immunodominant H-2Db-restricted CTL epitope of human PSA

Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet

Standard

Identification of an immunodominant H-2Db-restricted CTL epitope of human PSA. / Pavlenko, Maxim ; Leder, Christoph; Roos, Anna-Karin et al.
in: The Prostate, Jahrgang 64, Nr. 1, 15.06.2005, S. 50-59.

Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet

Harvard

Pavlenko, M, Leder, C, Roos, A-K, Levitsky, V & Pisa, P 2005, 'Identification of an immunodominant H-2Db-restricted CTL epitope of human PSA', The Prostate, Jg. 64, Nr. 1, S. 50-59. https://doi.org/10.1002/pros.20221

APA

Pavlenko, M., Leder, C., Roos, A.-K., Levitsky, V., & Pisa, P. (2005). Identification of an immunodominant H-2Db-restricted CTL epitope of human PSA. The Prostate, 64(1), 50-59. https://doi.org/10.1002/pros.20221

Vancouver

Pavlenko M, Leder C, Roos AK, Levitsky V, Pisa P. Identification of an immunodominant H-2Db-restricted CTL epitope of human PSA. The Prostate. 2005 Jun 15;64(1):50-59. doi: 10.1002/pros.20221

Bibtex

@article{09db3006aa2344a6aa52861dd0629258,
title = "Identification of an immunodominant H-2Db-restricted CTL epitope of human PSA",
abstract = "BACKGROUND. Human prostate specific antigen (PSA) is expressed selectively in prostate epithelium and is a potential target for the immunotherapy against prostate cancer. Various PSA-based vaccines have been reported to induce cytotoxic T lymphocyte (CTL) responses in animal models. Here, we present the identification and validation of an immunodominant CTL epitope of PSA in C57B1/6 mice (H-2 b). METHODS. PSA-specific CTLs were induced by immunization with a plasmid expressing PSA. Epitope specificity of the CTLs was determined by their reactivity against a panel of C-terminus truncated or mutated PSA proteins and use of bioinformatical prediction with the SYFPEITHI algorithm. RESULTS. The majority of PSA-specific CTLs were directed against a single H-2D b restricted epitope corresponding to the amino acid residues 65-74 (HCIRNKSVIL) of the protein. The CTLs had similar functional avidity against two putative H-2D b binding peptides: a 9-aa-long psa65-73 (HCIRNKSVI) and a 10-aa-long psa65-74 (HCIRNKSVIL). CONCLUSIONS. We demonstrate that the psa65-73 peptide can be used for reactivation of PSA-specific CTLs in vitro and ex vivo, and H-2D b pentamers assembled with this peptide are an efficient tool for monitoring of PSA-specific CTL responses after DNA vaccination.",
keywords = "Biology, prostate-specific antigen, DNA vaccine, CTL epitope, Immunodominance",
author = "Maxim Pavlenko and Christoph Leder and Anna-Karin Roos and V. Levitsky and Pavel Pisa",
year = "2005",
month = jun,
day = "15",
doi = "10.1002/pros.20221",
language = "English",
volume = "64",
pages = "50--59",
journal = "The Prostate",
issn = "0270-4137",
publisher = "John Wiley & Sons Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - Identification of an immunodominant H-2Db-restricted CTL epitope of human PSA

AU - Pavlenko, Maxim

AU - Leder, Christoph

AU - Roos, Anna-Karin

AU - Levitsky, V.

AU - Pisa, Pavel

PY - 2005/6/15

Y1 - 2005/6/15

N2 - BACKGROUND. Human prostate specific antigen (PSA) is expressed selectively in prostate epithelium and is a potential target for the immunotherapy against prostate cancer. Various PSA-based vaccines have been reported to induce cytotoxic T lymphocyte (CTL) responses in animal models. Here, we present the identification and validation of an immunodominant CTL epitope of PSA in C57B1/6 mice (H-2 b). METHODS. PSA-specific CTLs were induced by immunization with a plasmid expressing PSA. Epitope specificity of the CTLs was determined by their reactivity against a panel of C-terminus truncated or mutated PSA proteins and use of bioinformatical prediction with the SYFPEITHI algorithm. RESULTS. The majority of PSA-specific CTLs were directed against a single H-2D b restricted epitope corresponding to the amino acid residues 65-74 (HCIRNKSVIL) of the protein. The CTLs had similar functional avidity against two putative H-2D b binding peptides: a 9-aa-long psa65-73 (HCIRNKSVI) and a 10-aa-long psa65-74 (HCIRNKSVIL). CONCLUSIONS. We demonstrate that the psa65-73 peptide can be used for reactivation of PSA-specific CTLs in vitro and ex vivo, and H-2D b pentamers assembled with this peptide are an efficient tool for monitoring of PSA-specific CTL responses after DNA vaccination.

AB - BACKGROUND. Human prostate specific antigen (PSA) is expressed selectively in prostate epithelium and is a potential target for the immunotherapy against prostate cancer. Various PSA-based vaccines have been reported to induce cytotoxic T lymphocyte (CTL) responses in animal models. Here, we present the identification and validation of an immunodominant CTL epitope of PSA in C57B1/6 mice (H-2 b). METHODS. PSA-specific CTLs were induced by immunization with a plasmid expressing PSA. Epitope specificity of the CTLs was determined by their reactivity against a panel of C-terminus truncated or mutated PSA proteins and use of bioinformatical prediction with the SYFPEITHI algorithm. RESULTS. The majority of PSA-specific CTLs were directed against a single H-2D b restricted epitope corresponding to the amino acid residues 65-74 (HCIRNKSVIL) of the protein. The CTLs had similar functional avidity against two putative H-2D b binding peptides: a 9-aa-long psa65-73 (HCIRNKSVI) and a 10-aa-long psa65-74 (HCIRNKSVIL). CONCLUSIONS. We demonstrate that the psa65-73 peptide can be used for reactivation of PSA-specific CTLs in vitro and ex vivo, and H-2D b pentamers assembled with this peptide are an efficient tool for monitoring of PSA-specific CTL responses after DNA vaccination.

KW - Biology

KW - prostate-specific antigen

KW - DNA vaccine

KW - CTL epitope

KW - Immunodominance

UR - http://www.scopus.com/inward/record.url?scp=19544385574&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/07d6a316-3f31-3ee6-bf1b-b6cb18bffe15/

U2 - 10.1002/pros.20221

DO - 10.1002/pros.20221

M3 - Journal articles

VL - 64

SP - 50

EP - 59

JO - The Prostate

JF - The Prostate

SN - 0270-4137

IS - 1

ER -

DOI

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