HPLC and chemometrics-assisted UV-spectroscopy methods for the simultaneous determination of ambroxol and doxycycline in capsule.
Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung
Standard
HPLC and chemometrics-assisted UV-spectroscopy methods for the simultaneous determination of ambroxol and doxycycline in capsule. / Hadad, Ghada M.; El-Gindy, Alaa; Mahmoud, Waleed M. M.
in: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Jahrgang 70, Nr. 3, 08.2008, S. 655-663.Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - HPLC and chemometrics-assisted UV-spectroscopy methods for the simultaneous determination of ambroxol and doxycycline in capsule.
AU - Hadad, Ghada M.
AU - El-Gindy, Alaa
AU - Mahmoud, Waleed M. M.
PY - 2008/8
Y1 - 2008/8
N2 - High-performance liquid chromatography (HPLC) and multivariate spectrophotometric methods are described for the simultaneous determination of ambroxol hydrochloride (AM) and doxycycline (DX) in combined pharmaceutical capsules. The chromatographic separation was achieved on reversed-phase C(18) analytical column with a mobile phase consisting of a mixture of 20mM potassium dihydrogen phosphate, pH 6-acetonitrile in ratio of (1:1, v/v) and UV detection at 245 nm. Also, the resolution has been accomplished by using numerical spectrophotometric methods as classical least squares (CLS), principal component regression (PCR) and partial least squares (PLS-1) applied to the UV spectra of the mixture and graphical spectrophotometric method as first derivative of the ratio spectra ((1)DD) method. Analytical figures of merit (FOM), such as sensitivity, selectivity, analytical sensitivity, limit of quantitation and limit of detection were determined for CLS, PLS-1 and PCR methods. The proposed methods were validated and successfully applied for the analysis of pharmaceutical formulation and laboratory-prepared mixtures containing the two component combination.
AB - High-performance liquid chromatography (HPLC) and multivariate spectrophotometric methods are described for the simultaneous determination of ambroxol hydrochloride (AM) and doxycycline (DX) in combined pharmaceutical capsules. The chromatographic separation was achieved on reversed-phase C(18) analytical column with a mobile phase consisting of a mixture of 20mM potassium dihydrogen phosphate, pH 6-acetonitrile in ratio of (1:1, v/v) and UV detection at 245 nm. Also, the resolution has been accomplished by using numerical spectrophotometric methods as classical least squares (CLS), principal component regression (PCR) and partial least squares (PLS-1) applied to the UV spectra of the mixture and graphical spectrophotometric method as first derivative of the ratio spectra ((1)DD) method. Analytical figures of merit (FOM), such as sensitivity, selectivity, analytical sensitivity, limit of quantitation and limit of detection were determined for CLS, PLS-1 and PCR methods. The proposed methods were validated and successfully applied for the analysis of pharmaceutical formulation and laboratory-prepared mixtures containing the two component combination.
KW - Chemistry
KW - HPLC ANALYSIS
KW - Ambroxol
KW - Doxycyclin
UR - http://www.scopus.com/inward/record.url?scp=44649142496&partnerID=8YFLogxK
U2 - 10.1016/j.saa.2007.08.016
DO - 10.1016/j.saa.2007.08.016
M3 - Journal articles
C2 - 17931962
VL - 70
SP - 655
EP - 663
JO - Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
SN - 1386-1425
IS - 3
ER -