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Bioassay-directed chemical analysis utilizing LC-MS: a tool for identifying estrogenic compounds in water samples?

Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet

Standard

Bioassay-directed chemical analysis utilizing LC-MS: a tool for identifying estrogenic compounds in water samples? / Heisterkamp, Inga; Gandrass, Jürgen; Ruck, Wolfgang.
in: Analytical & Bioanalytical Chemistry, Jahrgang 378, Nr. 3, 01.02.2004, S. 709-715.

Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet

Harvard

Heisterkamp, I, Gandrass, J & Ruck, W 2004, 'Bioassay-directed chemical analysis utilizing LC-MS: a tool for identifying estrogenic compounds in water samples?', Analytical & Bioanalytical Chemistry, Jg. 378, Nr. 3, S. 709-715. https://doi.org/10.1007/s00216-003-2380-5

APA

Heisterkamp, I., Gandrass, J., & Ruck, W. (2004). Bioassay-directed chemical analysis utilizing LC-MS: a tool for identifying estrogenic compounds in water samples? Analytical & Bioanalytical Chemistry, 378(3), 709-715. https://doi.org/10.1007/s00216-003-2380-5

Vancouver

Heisterkamp I, Gandrass J, Ruck W. Bioassay-directed chemical analysis utilizing LC-MS: a tool for identifying estrogenic compounds in water samples? Analytical & Bioanalytical Chemistry. 2004 Feb 1;378(3):709-715. doi: 10.1007/s00216-003-2380-5

Bibtex

@article{9fd1a57adce7453bbbad2f5b6a2a5c9f,
title = "Bioassay-directed chemical analysis utilizing LC-MS: a tool for identifying estrogenic compounds in water samples?",
abstract = "A bioassay-directed chemical analysis (BDCA) scheme has been developed which combines a yeast screen for estrogenic activity with LC-MS detection after liquid-liquid extraction and fractionation by size exclusion chromatography. Focusing on sewage-treatment plant (STP) effluents, the approach aims at characterizing the substances responsible for estrogenic effects in aquatic systems. Initial results show a strong response of STP effluent extracts in the yeast screen. Estrone, bisphenol A, and nonylphenol have been identified as substances being partly responsible for observed estrogenic activity. However, confirmation experiments with synthetic samples revealed that the estrogenic effect potentials of the samples could not be completely assigned to specific compounds. Further improvement of the limits of detection of the analytical scheme is needed to enable identification and quantification of potent estrogenic compounds at low concentrations.",
keywords = "Chemistry, Bioassay-directed chemical analysis, Estrogenic activity, LC-MS-MS, Liquid-liquid extraction, Saccharomyces cerevisiae, Sewage-treatment plant effluent, Bioassay-directed chemical analysis, Estrogenic activity, LC-MS-MS, Liquid-liquid extraction, Saccharomyces cerevisiae, Sewage-treatment plant effluent",
author = "Inga Heisterkamp and J{\"u}rgen Gandrass and Wolfgang Ruck",
year = "2004",
month = feb,
day = "1",
doi = "10.1007/s00216-003-2380-5",
language = "English",
volume = "378",
pages = "709--715",
journal = "Analytical & Bioanalytical Chemistry",
issn = "1618-2642",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "3",

}

RIS

TY - JOUR

T1 - Bioassay-directed chemical analysis utilizing LC-MS: a tool for identifying estrogenic compounds in water samples?

AU - Heisterkamp, Inga

AU - Gandrass, Jürgen

AU - Ruck, Wolfgang

PY - 2004/2/1

Y1 - 2004/2/1

N2 - A bioassay-directed chemical analysis (BDCA) scheme has been developed which combines a yeast screen for estrogenic activity with LC-MS detection after liquid-liquid extraction and fractionation by size exclusion chromatography. Focusing on sewage-treatment plant (STP) effluents, the approach aims at characterizing the substances responsible for estrogenic effects in aquatic systems. Initial results show a strong response of STP effluent extracts in the yeast screen. Estrone, bisphenol A, and nonylphenol have been identified as substances being partly responsible for observed estrogenic activity. However, confirmation experiments with synthetic samples revealed that the estrogenic effect potentials of the samples could not be completely assigned to specific compounds. Further improvement of the limits of detection of the analytical scheme is needed to enable identification and quantification of potent estrogenic compounds at low concentrations.

AB - A bioassay-directed chemical analysis (BDCA) scheme has been developed which combines a yeast screen for estrogenic activity with LC-MS detection after liquid-liquid extraction and fractionation by size exclusion chromatography. Focusing on sewage-treatment plant (STP) effluents, the approach aims at characterizing the substances responsible for estrogenic effects in aquatic systems. Initial results show a strong response of STP effluent extracts in the yeast screen. Estrone, bisphenol A, and nonylphenol have been identified as substances being partly responsible for observed estrogenic activity. However, confirmation experiments with synthetic samples revealed that the estrogenic effect potentials of the samples could not be completely assigned to specific compounds. Further improvement of the limits of detection of the analytical scheme is needed to enable identification and quantification of potent estrogenic compounds at low concentrations.

KW - Chemistry

KW - Bioassay-directed chemical analysis

KW - Estrogenic activity

KW - LC-MS-MS

KW - Liquid-liquid extraction

KW - Saccharomyces cerevisiae

KW - Sewage-treatment plant effluent

KW - Bioassay-directed chemical analysis

KW - Estrogenic activity

KW - LC-MS-MS

KW - Liquid-liquid extraction

KW - Saccharomyces cerevisiae

KW - Sewage-treatment plant effluent

UR - https://www.mendeley.com/catalogue/e07bbd21-06ad-3619-9e7f-fc52070949cc/

UR - http://www.scopus.com/inward/record.url?scp=1842463404&partnerID=8YFLogxK

U2 - 10.1007/s00216-003-2380-5

DO - 10.1007/s00216-003-2380-5

M3 - Journal articles

VL - 378

SP - 709

EP - 715

JO - Analytical & Bioanalytical Chemistry

JF - Analytical & Bioanalytical Chemistry

SN - 1618-2642

IS - 3

ER -

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DOI

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