Bioassay-directed chemical analysis utilizing LC-MS: a tool for identifying estrogenic compounds in water samples?
Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet
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in: Analytical & Bioanalytical Chemistry, Jahrgang 378, Nr. 3, 01.02.2004, S. 709-715.
Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet
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TY - JOUR
T1 - Bioassay-directed chemical analysis utilizing LC-MS: a tool for identifying estrogenic compounds in water samples?
AU - Heisterkamp, Inga
AU - Gandrass, Jürgen
AU - Ruck, Wolfgang
PY - 2004/2/1
Y1 - 2004/2/1
N2 - A bioassay-directed chemical analysis (BDCA) scheme has been developed which combines a yeast screen for estrogenic activity with LC-MS detection after liquid-liquid extraction and fractionation by size exclusion chromatography. Focusing on sewage-treatment plant (STP) effluents, the approach aims at characterizing the substances responsible for estrogenic effects in aquatic systems. Initial results show a strong response of STP effluent extracts in the yeast screen. Estrone, bisphenol A, and nonylphenol have been identified as substances being partly responsible for observed estrogenic activity. However, confirmation experiments with synthetic samples revealed that the estrogenic effect potentials of the samples could not be completely assigned to specific compounds. Further improvement of the limits of detection of the analytical scheme is needed to enable identification and quantification of potent estrogenic compounds at low concentrations.
AB - A bioassay-directed chemical analysis (BDCA) scheme has been developed which combines a yeast screen for estrogenic activity with LC-MS detection after liquid-liquid extraction and fractionation by size exclusion chromatography. Focusing on sewage-treatment plant (STP) effluents, the approach aims at characterizing the substances responsible for estrogenic effects in aquatic systems. Initial results show a strong response of STP effluent extracts in the yeast screen. Estrone, bisphenol A, and nonylphenol have been identified as substances being partly responsible for observed estrogenic activity. However, confirmation experiments with synthetic samples revealed that the estrogenic effect potentials of the samples could not be completely assigned to specific compounds. Further improvement of the limits of detection of the analytical scheme is needed to enable identification and quantification of potent estrogenic compounds at low concentrations.
KW - Chemistry
KW - Bioassay-directed chemical analysis
KW - Estrogenic activity
KW - LC-MS-MS
KW - Liquid-liquid extraction
KW - Saccharomyces cerevisiae
KW - Sewage-treatment plant effluent
KW - Bioassay-directed chemical analysis
KW - Estrogenic activity
KW - LC-MS-MS
KW - Liquid-liquid extraction
KW - Saccharomyces cerevisiae
KW - Sewage-treatment plant effluent
UR - https://www.mendeley.com/catalogue/e07bbd21-06ad-3619-9e7f-fc52070949cc/
UR - http://www.scopus.com/inward/record.url?scp=1842463404&partnerID=8YFLogxK
U2 - 10.1007/s00216-003-2380-5
DO - 10.1007/s00216-003-2380-5
M3 - Journal articles
VL - 378
SP - 709
EP - 715
JO - Analytical & Bioanalytical Chemistry
JF - Analytical & Bioanalytical Chemistry
SN - 1618-2642
IS - 3
ER -