TY - JOUR

T1 - Application of CEICPMS and CEESIMS in metalloproteomics

T2 - challenges, developments, and limitations

AU - Pröfrock, Daniel

AU - Prange, Andreas

PY - 2005/10

Y1 - 2005/10

N2 - Application of capillary electrophoresis (CE) as a high-resolution separation technique in metalloproteomics research is critically reviewed. The focus is on the requirements and challenges involved in coupling CE to sensitive element and molecule-specific detection techniques such as inductively coupled plasma mass spectrometry (ICP-MS) or electrospray ionisation mass spectrometry (ESI-MS). The complementary application of both detection techniques to the structural and functional characterisation of metal-binding proteins and their structural metal-binding moieties is emphasised. Beneficial aspects and limitations of mass spectrometry hyphenated to CE are discussed, on the basis of the literature published in this field over the last decade. Recent metalloproteomics applications of CE are reviewed to demonstrate its potential and limitations in modern biochemical speciation analysis and to indicate future directions of this technique.

AB - Application of capillary electrophoresis (CE) as a high-resolution separation technique in metalloproteomics research is critically reviewed. The focus is on the requirements and challenges involved in coupling CE to sensitive element and molecule-specific detection techniques such as inductively coupled plasma mass spectrometry (ICP-MS) or electrospray ionisation mass spectrometry (ESI-MS). The complementary application of both detection techniques to the structural and functional characterisation of metal-binding proteins and their structural metal-binding moieties is emphasised. Beneficial aspects and limitations of mass spectrometry hyphenated to CE are discussed, on the basis of the literature published in this field over the last decade. Recent metalloproteomics applications of CE are reviewed to demonstrate its potential and limitations in modern biochemical speciation analysis and to indicate future directions of this technique.

KW - Chemistry

KW - Capillary electrophoresis

KW - ESI-MS

KW - HR-ICP-MS

KW - Hyphenated techniques

KW - ICP-MS

KW - Interface

KW - Metallomics

KW - Metalloproteomics

KW - Metallothioneine

KW - Nebuliser

KW - Proteins

KW - Selenoprotein

UR - http://www.scopus.com/inward/record.url?scp=27144491183&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/45d34162-6234-36f8-bbcb-152006505e7e/

U2 - 10.1007/s00216-005-3420-0

DO - 10.1007/s00216-005-3420-0

M3 - Scientific review articles

VL - 383

SP - 372

EP - 389

JO - Analytical & Bioanalytical Chemistry

JF - Analytical & Bioanalytical Chemistry

SN - 1618-2642

IS - 3

ER -