A potassium channel protein encoded by chlorella virus PBCV-1

Publikationen: Beiträge in ZeitschriftenZeitschriftenaufsätze

Authors

  • Barbara Plugge
  • S Gazzarrini
  • M Nelson
  • R Cerana
  • J L Van Etten
  • C Derst
  • D DiFrancesco
  • A Moroni
  • G Thiel
The large chlorella virus PBCV-1, which contains double-stranded DNA (dsDNA), encodes a 94-codon open reading frame (ORF) that contains a motif resembling the signature sequence of the pore domain of potassium channel proteins. Phylogenetic analyses of the encoded protein, Kcv, indicate a previously unidentified type of potassium channel. The messenger RNA encoded by the ORF leads to functional expression of a potassium-selective conductance in Xenopus laevis oocytes. The channel blockers amantadine and barium, but not cesium, inhibit this conductance, in addition to virus plaque formation. Thus, PBCV-1 encodes the first known viral protein that functions as a potassium-selective channel and is essential in the virus life cycle.
OriginalspracheEnglisch
ZeitschriftScience
Band287
Ausgabennummer5458
Seiten (von - bis)1641-1644
Anzahl der Seiten4
ISSN0036-8075
DOIs
PublikationsstatusErschienen - 03.03.2000

DOI

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