TY - JOUR

T1 - Range-wide genetic structure and diversity of the endemic tree line species Polylepis australis (Rosaceae) in Argentina

AU - Hensen, I.

AU - Teich, Ingrid

AU - Hirsch, H.

AU - von Wehrden, H.

AU - Renison, D.

N1 - Export Date: 21 May 2012 Source: Scopus CODEN: AJBOA doi: 10.3732/ajb.1000100 Language of Original Document: English Correspondence Address: Hensen, I.; Institute of Biology/Geobotany and Botanical Garden, Martin-Luther-University Halle-Wittenberg, Am Kirchtor 1, D-06108 Halle, Germany; email: isabell.hensen@botanik.uni-halle.de References: Behling, H., Berrio, J.C., Hooghiemstra, H., Late Quaternary pollen records from the middle Caquetáriver basin in central Colombian Amazon (1999) Palaeogeography, Palaeoclimatology, Palaeoecology, 145, pp. 193-213; Bekessy, S.A., Allnutt, T.R., Premoli, A.C., Lara, A., Ennos, R.A., Burgman, M.A., Cortes, M., Newton, A.C., Genetic variation in the vulnerable and endemic monkey puzzle tree, detected using RAPDs (2002) Heredity, 88, pp. 243-249; Bonin, A., Ehrich, D., Manel, S., Statistical analysis of amplified fragment length polymorphism data: A toolbox for molecular ecologists and evolutionists (2007) Molecular Ecology, 16, pp. 3737-3758; Bush, M.B., Hansen, B.S.C., Rodbell, D.T., Seltzer, G.O., Young, K.R., León, B., Abbott, M.B., A 1700.0-year history of Andean climate and vegetation change from Laguna de Chochos, Peru (2005) Journal of Quaternary Science, 20, pp. 703-714; Cao, C.-P., Gailing, O., Siregar, I.Z., Siregar, U.J., Finkeldey, R., Genetic variation in nine Shorea species (Dipterocarpaceae) in Indonesia revealed by AFLPs (2009) Tree Genetics & Genomes, 5, pp. 407-420; Cierjacks, A., Rühr, N., Wesche, K., Hensen, I., Effects of altitude and livestock on the regeneration of two tree line forming Polylepis species in Ecuador (2008) Plant Ecology, 194, pp. 207-221; Cingolani, A., Renison, D., Tecco, P., Gurvich, D., Cabido, M., Predicting cover types in a mountain range with long evolutionary grazing history: A GIS approach (2008) Journal of Biogeography, 35, pp. 538-551; Colinvaux, P.A., Bush, M.B., Steinitz-Kannan, M., Miller, M.C., Glacial and postglacial pollen records from the Ecuadorian Andes and Amazon (1997) Quaternary Research, 48, pp. 69-78; Colinvaux, P.A., de Oliveira, P.E., Moreno, J.E., Miller, M.C., Bush, M.B., A long pollen record from lowland Amazonia: Forest and cooling in glacial times (1996) Science, 274, pp. 85-87; Colinvaux, P.A., Liu, K.-B., de Oliveira, P.E., Bush, M.B., Miller, M.C., Steinitz-Kannan, M., Temperature depression in the lowland tropics in glacial times (1996) Climatic Change, 32, pp. 19-33; di Pasquale, G., Marziano, M., Impagliazzo, S., Lubritto, C., de Natale, A., Bader, M.Y., The holocene treeline in the northern Andes (Ecuador): First evidence from soil charcoal (2008) Palaeogeography, Palaeoclimatology, Palaeoecology, 259, pp. 17-34; Doyle, J.J., Doyle, J.L., A rapid DNA isolation procedure for small quantities of fresh leaf tissue (1987) Phytochemical Bulletin, 19, pp. 11-15; Evanno, G., Regnaut, S., Goudet, J., Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study (2005) Molecular Ecology, 14, pp. 2611-2620; Falush, D., Stephens, M., Pritchard, J.K., Inference of population structure using multilocus genotype data: Dominant markers and null alleles (2007) Molecular Ecology, 7, pp. 574-578; Gosling, W.D., Hanselman, J.A., Knox, C., Valencia, B.G., Bush, M.B., Long-term drivers of change in Polylepis woodland distribution in the central Andes (2009) Journal of Vegetation Science, 20, pp. 1041-1052; Hamrick, J.L., Response of forest trees to global environmental changes (2004) Forest Ecology and Management, 197, pp. 323-335; Hamrick, J.L., Godt, M.J.W., Sherman-Broyles, S.L., Factors influencing levels of genetic diversity in woody plant species (1992) New Forests, 6, pp. 95-124; Hanselman, J.A., Gosling, W.D., Paduano, G.M., Bush, M.B., Contrasting pollen histories of MIS 5e and the Holocene from Lake Titicaca (Bolivia/Peru) (2005) Journal of Quaternary Science, 20, pp. 663-670; Hansen, B.C.S., Rodbell, D.T., Seltzer, G.O., León, B., Young, K.R., Abbott, M., Late-glacial and Holocene vegetational history from two sites in the western Cordillera of southwestern Ecuador (2003) Palaeogeography, Palaeoclimatology, Palaeoecology, 194, pp. 79-108; Haselton, K., Hilley, G., Strecker, M.R., Average Pleistocene climatic patterns in the Southern Central Andes: Controls on mountain glaciation and paleoclimate implications (2002) Journal of Geology, 110, pp. 211-226; Hensen, I., Cierjacks, A., Hirsch, H., Kessler, M., Romoleroux, K., Renison, D., Wesche, K., Historic and recent fragmentation coupled with altitude affect the genetic population structure of one of the world' s highest tropical tree line species (2011) Global Ecology and Biogeography; Heuertz, M., Hausman, J.-F., Tsvetkov, I., Frascaria-Lacoste, N., Vekemans, X., Assessment of genetic structure within and among Bulgarian populations of the common ash (Fraxinus excelsior L.) 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PY - 2011/11/11

Y1 - 2011/11/11

N2 - Premise of the study: Knowledge on the range-wide distribution of genetic structure and diversity is required to facilitate the understanding of historical tree migration and for predicting responses to current climate change. With respect to post-glacial migration patterns known from the northern hemisphere, we tested the prediction that the southernmost populations of a subtropical tree line species have lower within-population genetic diversity and higher genetic differentiation than the central and northernmost populations. Methods: We used AFLP to assess the genetic structure of 18 populations of the wind-pollinated Polylepis australis (Rosaceae) sampled over its entire distributional range in three Argentinean high mountain regions. Genetic diversity was calculated as a percentage of polymorphic bands (P) and Nei's expected heterozygosity (He); genetic differentiation was assessed using AMOVA, Φ ST - statistics, and Bayesian cluster analysis. Key results: Contrary to our expectations, the northernmost Polylepis australis stands had lower within-population genetic diversity and higher genetic differentiation than the central and southernmost stands. Populations grouped into two major clusters, the first containing the southern populations and four central populations and the second containing the northern and one central population. Conclusions: Patterns of Polylepis australis genetic structure and diversity differ from historical migration scenarios observed for the northern hemisphere. The decline in genetic diversity toward the north may point to an equatorward migration following past climatic changes. Populations within the south and central part appear to be connected by effective long-distance pollination while gene flow in the northern part is probably hampered by geographic isolation.

AB - Premise of the study: Knowledge on the range-wide distribution of genetic structure and diversity is required to facilitate the understanding of historical tree migration and for predicting responses to current climate change. With respect to post-glacial migration patterns known from the northern hemisphere, we tested the prediction that the southernmost populations of a subtropical tree line species have lower within-population genetic diversity and higher genetic differentiation than the central and northernmost populations. Methods: We used AFLP to assess the genetic structure of 18 populations of the wind-pollinated Polylepis australis (Rosaceae) sampled over its entire distributional range in three Argentinean high mountain regions. Genetic diversity was calculated as a percentage of polymorphic bands (P) and Nei's expected heterozygosity (He); genetic differentiation was assessed using AMOVA, Φ ST - statistics, and Bayesian cluster analysis. Key results: Contrary to our expectations, the northernmost Polylepis australis stands had lower within-population genetic diversity and higher genetic differentiation than the central and southernmost stands. Populations grouped into two major clusters, the first containing the southern populations and four central populations and the second containing the northern and one central population. Conclusions: Patterns of Polylepis australis genetic structure and diversity differ from historical migration scenarios observed for the northern hemisphere. The decline in genetic diversity toward the north may point to an equatorward migration following past climatic changes. Populations within the south and central part appear to be connected by effective long-distance pollination while gene flow in the northern part is probably hampered by geographic isolation.

KW - Ecosystems Research

KW - AFLP

KW - Climate change

KW - High mountain forests

KW - Neotropics

KW - Population genetics

KW - Tree migration

KW - climate change

KW - cluster analysis

KW - dicotyledon

KW - endemic species

KW - gene flow

KW - genetic variation

KW - migration route

KW - montane forest

KW - Neotropical Region

KW - Northern Hemisphere

KW - polymorphism

KW - Postglacial

KW - Argentina

UR - http://www.scopus.com/inward/record.url?scp=84858433251&partnerID=8YFLogxK

U2 - 10.3732/ajb.1000100

DO - 10.3732/ajb.1000100

M3 - Journal articles

C2 - 22034483

VL - 98

SP - 1825

EP - 1833

JO - American Journal of Botany

JF - American Journal of Botany

SN - 0002-9122

IS - 11

ER -