Effects of pesticide application and plant sexual identity on leaf physiological traits and phyllosphere bacterial communities

Zuodong Zhu; Yue He; Jiahui Xu; Zhenghu Zhou; Amit Kumar; Zhichao Xia

doi:10.1093/jpe/rtac084

Effects of pesticide application and plant sexual identity on leaf physiological traits and phyllosphere bacterial communities

Research output: Journal contributions › Journal articles › Research

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Effects of pesticide application and plant sexual identity on leaf physiological traits and phyllosphere bacterial communities. / Zhu, Zuodong; He, Yue; Xu, Jiahui et al.
In: Journal of Plant Ecology, Vol. 16, No. 2, rtac084, 01.04.2023.

Research output: Journal contributions › Journal articles › Research

Bibtex

@article{315d7436200e468e8c2187dca88d4d01,

title = "Effects of pesticide application and plant sexual identity on leaf physiological traits and phyllosphere bacterial communities",

abstract = "Pesticides are widely used to enhance food production on a global scale. However, little information is available on the effects of pesticide application on leaf physiology and phyllosphere bacterial communities of dioecious plants. Therefore, this study aimed to assess the impact of λ-cyhalothrin, a broad-spectrum pesticide, on leaf physiology and phyllosphere bacterial communities in the dioecious Populus cathayana. Physiological leaf traits such as photosynthetic apparatus (net photosynthetic rate (Pn), stomatal conductance (gs) and transpiration (E)) of males were significantly higher than those of females, independent of pesticide use. In contrast, pesticide application significantly reduced the photosynthetic apparatus for both sexes, and the reduction was greater in males relative to females. Also, pesticide application significantly increased peroxidase (POD) activity and malondialdehyde (MDA) content and maintained superoxide dismutase (SOD) activity and total chlorophyll content in leaves of males. The phyllosphere bacteria showed some conserved characteristics, in which, Simpson and Shannon diversity indices were not affected by sex or pesticide application. Phyllosphere bacterial community composition differed between females and males, indicating that intrinsic sex significantly shapes the phyllosphere bacterial community. However, pesticide application significantly increased the relative abundance of Actinobacteria but reduced the relative abundance of Proteobacteria. Principal component analysis showed associations between leaf physiology and specific bacterial taxa. For instance, Proteobacteria negatively correlated with leaf SOD activity and MDA content, while Actinobacteria showed an opposite pattern. Our study highlights sex-specific phyllosphere bacterial community composition and leaf physiological traits in dioecious plants.",

keywords = "Biology, dioecious plants, λ-cyhalothrin, leaf physiology, phyllosphere bacterial community, Sex-specific responses",

author = "Zuodong Zhu and Yue He and Jiahui Xu and Zhenghu Zhou and Amit Kumar and Zhichao Xia",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s). Published by Oxford University Press on behalf of the Institute of Botany, Chinese Academy of Sciences and the Botanical Society of China.",

year = "2023",

month = apr,

day = "1",

doi = "10.1093/jpe/rtac084",

language = "English",

volume = "16",

journal = "Journal of Plant Ecology",

issn = "1752-9921",

publisher = "Oxford University Press",

number = "2",

}

RIS

TY - JOUR

T1 - Effects of pesticide application and plant sexual identity on leaf physiological traits and phyllosphere bacterial communities

AU - Zhu, Zuodong

AU - He, Yue

AU - Xu, Jiahui

AU - Zhou, Zhenghu

AU - Kumar, Amit

AU - Xia, Zhichao

PY - 2023/4/1

Y1 - 2023/4/1

N2 - Pesticides are widely used to enhance food production on a global scale. However, little information is available on the effects of pesticide application on leaf physiology and phyllosphere bacterial communities of dioecious plants. Therefore, this study aimed to assess the impact of λ-cyhalothrin, a broad-spectrum pesticide, on leaf physiology and phyllosphere bacterial communities in the dioecious Populus cathayana. Physiological leaf traits such as photosynthetic apparatus (net photosynthetic rate (Pn), stomatal conductance (gs) and transpiration (E)) of males were significantly higher than those of females, independent of pesticide use. In contrast, pesticide application significantly reduced the photosynthetic apparatus for both sexes, and the reduction was greater in males relative to females. Also, pesticide application significantly increased peroxidase (POD) activity and malondialdehyde (MDA) content and maintained superoxide dismutase (SOD) activity and total chlorophyll content in leaves of males. The phyllosphere bacteria showed some conserved characteristics, in which, Simpson and Shannon diversity indices were not affected by sex or pesticide application. Phyllosphere bacterial community composition differed between females and males, indicating that intrinsic sex significantly shapes the phyllosphere bacterial community. However, pesticide application significantly increased the relative abundance of Actinobacteria but reduced the relative abundance of Proteobacteria. Principal component analysis showed associations between leaf physiology and specific bacterial taxa. For instance, Proteobacteria negatively correlated with leaf SOD activity and MDA content, while Actinobacteria showed an opposite pattern. Our study highlights sex-specific phyllosphere bacterial community composition and leaf physiological traits in dioecious plants.

AB - Pesticides are widely used to enhance food production on a global scale. However, little information is available on the effects of pesticide application on leaf physiology and phyllosphere bacterial communities of dioecious plants. Therefore, this study aimed to assess the impact of λ-cyhalothrin, a broad-spectrum pesticide, on leaf physiology and phyllosphere bacterial communities in the dioecious Populus cathayana. Physiological leaf traits such as photosynthetic apparatus (net photosynthetic rate (Pn), stomatal conductance (gs) and transpiration (E)) of males were significantly higher than those of females, independent of pesticide use. In contrast, pesticide application significantly reduced the photosynthetic apparatus for both sexes, and the reduction was greater in males relative to females. Also, pesticide application significantly increased peroxidase (POD) activity and malondialdehyde (MDA) content and maintained superoxide dismutase (SOD) activity and total chlorophyll content in leaves of males. The phyllosphere bacteria showed some conserved characteristics, in which, Simpson and Shannon diversity indices were not affected by sex or pesticide application. Phyllosphere bacterial community composition differed between females and males, indicating that intrinsic sex significantly shapes the phyllosphere bacterial community. However, pesticide application significantly increased the relative abundance of Actinobacteria but reduced the relative abundance of Proteobacteria. Principal component analysis showed associations between leaf physiology and specific bacterial taxa. For instance, Proteobacteria negatively correlated with leaf SOD activity and MDA content, while Actinobacteria showed an opposite pattern. Our study highlights sex-specific phyllosphere bacterial community composition and leaf physiological traits in dioecious plants.

KW - Biology

KW - dioecious plants

KW - λ-cyhalothrin

KW - leaf physiology

KW - phyllosphere bacterial community

KW - Sex-specific responses

UR - http://www.scopus.com/inward/record.url?scp=85153373771&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/491717d8-aa29-3319-9bb2-d0fac5a6f019/

U2 - 10.1093/jpe/rtac084

DO - 10.1093/jpe/rtac084

M3 - Journal articles

VL - 16

JO - Journal of Plant Ecology

JF - Journal of Plant Ecology

SN - 1752-9921

IS - 2

M1 - rtac084

ER -

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