Application of CEICPMS and CEESIMS in metalloproteomics: challenges, developments, and limitations

Research output: Journal contributionsScientific review articlesResearch


Application of capillary electrophoresis (CE) as a high-resolution separation technique in metalloproteomics research is critically reviewed. The focus is on the requirements and challenges involved in coupling CE to sensitive element and molecule-specific detection techniques such as inductively coupled plasma mass spectrometry (ICP-MS) or electrospray ionisation mass spectrometry (ESI-MS). The complementary application of both detection techniques to the structural and functional characterisation of metal-binding proteins and their structural metal-binding moieties is emphasised. Beneficial aspects and limitations of mass spectrometry hyphenated to CE are discussed, on the basis of the literature published in this field over the last decade. Recent metalloproteomics applications of CE are reviewed to demonstrate its potential and limitations in modern biochemical speciation analysis and to indicate future directions of this technique.

Original languageEnglish
JournalAnalytical & Bioanalytical Chemistry
Issue number3
Pages (from-to)372-389
Number of pages18
Publication statusPublished - 10.2005

    Research areas

  • Chemistry
  • Capillary electrophoresis, ESI-MS, HR-ICP-MS, Hyphenated techniques, ICP-MS, Interface, Metallomics, Metalloproteomics, Metallothioneine, Nebuliser, Proteins, Selenoprotein