TY - JOUR

T1 - The reciprocal iso-inhibition volume concept

T2 - A procedure for the evaluation in effect-directed analysis with thin-layer chromatography - using the thin-layer chromatography-luminescent bacteria assay as an example

AU - Schulz, Wolfgang

AU - Weiss, Stefan C.

AU - Weber, Walter H.

AU - Winzenbacher, Rudi

PY - 2017/10/13

Y1 - 2017/10/13

N2 - In effect-directed analysis (EDA) with high-performance thin-layer chromatography (HPTLC), the effect is often detected using images. Thus, an approach to create inhibition chromatograms from these images was developed using the example of the HPTLC- bioluminescence inhibition test. A comparison between the cuvette test and the HPTLC test shows that the test on the plate is significantly more sensitive. To describe the strength of the effect, the EC50 value is determined from the dose-response relationship. However, the inhibiting compounds are generally unknown and thus their concentrations are also unknown. Therefore, instead of the concentration, the known application volumes are used. This enables the calculation of the application volume necessary to achieve 50% inhibition. Since the volume is inversely proportional to the concentration, the reciprocal value of the calculated volume is indicated and is referred to as the reciprocal iso-inhibition volume (RIV). Using this RIV-concept, it is now possible to compare inhibition bands within and between plates. The entire evaluation is described by the means of two samples from a contaminated site using the bioluminescence inhibition.

AB - In effect-directed analysis (EDA) with high-performance thin-layer chromatography (HPTLC), the effect is often detected using images. Thus, an approach to create inhibition chromatograms from these images was developed using the example of the HPTLC- bioluminescence inhibition test. A comparison between the cuvette test and the HPTLC test shows that the test on the plate is significantly more sensitive. To describe the strength of the effect, the EC50 value is determined from the dose-response relationship. However, the inhibiting compounds are generally unknown and thus their concentrations are also unknown. Therefore, instead of the concentration, the known application volumes are used. This enables the calculation of the application volume necessary to achieve 50% inhibition. Since the volume is inversely proportional to the concentration, the reciprocal value of the calculated volume is indicated and is referred to as the reciprocal iso-inhibition volume (RIV). Using this RIV-concept, it is now possible to compare inhibition bands within and between plates. The entire evaluation is described by the means of two samples from a contaminated site using the bioluminescence inhibition.

KW - Aliivibrio fischeri

KW - EDA

KW - Environmental analysis

KW - HPTLC

KW - HPTLC/AMD

KW - Chemistry

UR - http://www.scopus.com/inward/record.url?scp=85029713471&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2017.08.076

DO - 10.1016/j.chroma.2017.08.076

M3 - Journal articles

C2 - 28888680

AN - SCOPUS:85029713471

VL - 1519

SP - 121

EP - 130

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

ER -