This study aimed to investigate the variables determining performance in a simulated on-snow Skimo sprint competition, and how their relationship with performance evolves from the individual time trial to the final. Fifteen national-level junior Skimo athletes (mean ± SD: age, 17.8 ± 2.5 years; maximal oxygen uptake, 66.8 mL·kg⁻¹·min⁻¹) underwent a comprehensive assessment, involving submaximal and maximal endurance tests, maximal strength assessments, and a maximal sprint to determine maximal glycolytic capacity. Subsequently, a simulated sprint competition, comprising an individual time-trial and three heats (quarterfinal, semifinal, final), was conducted. Whole-body and upper body aerobic power (r = 0.69–0.93), maximal speed and power (r = 0.82–0.85) during the maximal performance test, as well as fat-free mass (r = 0.62–0.77) and body fat (r = −0.67–−0.77), exhibited significant correlations with performance in the time-trial, quarterfinal and semifinal. Moreover, maximal strength (r = 0.39–0.95) and transition duration (r = 0.52–0.85) showed moderate to large correlations with sprint performance. Overall, aerobic power, maximal speed and power, as well as fat-free mass, and body fat emerged as crucial determinants of Skimo sprint performance, while dynamic strength and the ability to transition quickly between sections also proved to be relevant factors.