TY - JOUR

T1 - Optimization and validation of an HPLC-UV method for determination of tranexamic acid in a dosage form and in human urine.

AU - Hadad, Ghada M.

AU - El-Gindy, Alaa

AU - Mahmoud, Waleed M. M.

PY - 2007/9

Y1 - 2007/9

N2 - A simple and reliable high-performance liquid chromatographic method with UV detection at 245 nm has been developed and validated for determination of tranexamic acid (TA) in a dosage form and in human urine. Before injection samples were derivatized with phenyl isothiocyanate (PITC). The reaction temperature, reaction time, and concentration of PITC used for derivatization were optimized. Chromatographic separation was on a C18 column with a 65:35 (v/v) mixture of 10 mM phosphate buffer, pH 3.6, and acetonitrile as mobile phase. Heptaminol hydrochloride was used as internal standard.

AB - A simple and reliable high-performance liquid chromatographic method with UV detection at 245 nm has been developed and validated for determination of tranexamic acid (TA) in a dosage form and in human urine. Before injection samples were derivatized with phenyl isothiocyanate (PITC). The reaction temperature, reaction time, and concentration of PITC used for derivatization were optimized. Chromatographic separation was on a C18 column with a 65:35 (v/v) mixture of 10 mM phosphate buffer, pH 3.6, and acetonitrile as mobile phase. Heptaminol hydrochloride was used as internal standard.

KW - Chemistry

KW - Column liquid chromatography

KW - Urinary excretion

KW - Tranexamic acid

UR - https://www.mendeley.com/catalogue/b1d31cd0-5893-3985-bf8e-f8c9b2eea928/

U2 - 10.1365/s10337-007-0323-6

DO - 10.1365/s10337-007-0323-6

M3 - Journal articles

VL - 66

SP - 311

EP - 317

JO - Chromatographia

JF - Chromatographia

SN - 0009-5893

IS - 5-6

ER -