Isolation of a Na v channel blocking polypeptide from Cyanea capillata medusae: A neurotoxin contained in fishing tentacle isorhizas
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Isolation of a Na v channel blocking polypeptide from Cyanea capillata medusae : A neurotoxin contained in fishing tentacle isorhizas. / Lassen, Stephan; Wiebring, Annika; Helmholz, Heike et al.
in: Toxicon, Jahrgang 59, Nr. 6, 05.2012, S. 610-616.Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet
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TY - JOUR
T1 - Isolation of a Na v channel blocking polypeptide from Cyanea capillata medusae
T2 - A neurotoxin contained in fishing tentacle isorhizas
AU - Lassen, Stephan
AU - Wiebring, Annika
AU - Helmholz, Heike
AU - Ruhnau, Christiane
AU - Prange, Andreas
PY - 2012/5
Y1 - 2012/5
N2 - Jellyfish are efficient predators which prey on crabs, fish larvae, and small fish. Their venoms consist of various toxins including neurotoxins that paralyse prey organisms immediately. One possible mode of action of neurotoxins is the blockage of voltage-gated sodium (Na v) channels. A novel polypeptide with Na v channel blocking activity was isolated from the northern Scyphozoa Cyanea capillata (L., 1758). For that purpose, a bioactivity-guided multidimensional liquid chromatographic purification method has been developed. A neurotoxic activity of resulting chromatographic fractions was demonstrated by a bioassay, which based on the mouse neuroblastoma cell line Neuro2A. The purification process yielded one fraction containing a single polypeptide with proven activity. The molecular weight of 8.22kDa was determined by matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-ToF MS). Utilising Laser Microdissection and Pressure Catapulting (LMPC) for the separation of different nematocyst types in combination with direct MALDI-ToF MS analysis of the intact capsules, the neurotoxin was found to be present in all types of fishing tentacle isorhizas (A-isorhizas, a-isorhizas, O-isorhizas) of C. capillata medusae.
AB - Jellyfish are efficient predators which prey on crabs, fish larvae, and small fish. Their venoms consist of various toxins including neurotoxins that paralyse prey organisms immediately. One possible mode of action of neurotoxins is the blockage of voltage-gated sodium (Na v) channels. A novel polypeptide with Na v channel blocking activity was isolated from the northern Scyphozoa Cyanea capillata (L., 1758). For that purpose, a bioactivity-guided multidimensional liquid chromatographic purification method has been developed. A neurotoxic activity of resulting chromatographic fractions was demonstrated by a bioassay, which based on the mouse neuroblastoma cell line Neuro2A. The purification process yielded one fraction containing a single polypeptide with proven activity. The molecular weight of 8.22kDa was determined by matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-ToF MS). Utilising Laser Microdissection and Pressure Catapulting (LMPC) for the separation of different nematocyst types in combination with direct MALDI-ToF MS analysis of the intact capsules, the neurotoxin was found to be present in all types of fishing tentacle isorhizas (A-isorhizas, a-isorhizas, O-isorhizas) of C. capillata medusae.
KW - Chemistry
KW - Cyanea
KW - Jellyfish
KW - Mass spectrometry
KW - Nematocysts
KW - Neurotoxin
UR - http://www.scopus.com/inward/record.url?scp=84857925857&partnerID=8YFLogxK
U2 - 10.1016/j.toxicon.2012.02.004
DO - 10.1016/j.toxicon.2012.02.004
M3 - Journal articles
C2 - 22402177
AN - SCOPUS:84857925857
VL - 59
SP - 610
EP - 616
JO - Toxicon
JF - Toxicon
SN - 0041-0101
IS - 6
ER -