TY - JOUR

T1 - High performance liquid chromatographic determination of etofibrate and its hydrolysis products.

AU - El-Gindy, A.

AU - Hadad, Ghada M.

AU - Mahmoud, Waleed M. M.

PY - 2007/1/4

Y1 - 2007/1/4

N2 - High performance liquid chromatographic (HPLC) method is presented for the determination of etofibrate (EF) and its hydrolysis products. The method was based on HPLC separation of EF from its hydrolysis products using cyanopropyl column at ambient temperature with mobile phase consisting of acetonitrile–10 mM potassium dihydrogen phosphate, pH was adjusted to 4.1 using phosphoric acid (50:50, v/v). Quantitation was achieved with UV detection at 221 nm based on peak area. The flow rate was 1.5 ml min−1. The proposed method was used to investigate the kinetics of acidic hydrolysis process of EF at different temperatures and the apparent pseudo first-order rate constant, half-life and activation energy were calculated. The kinetics of alkaline hydrolysis process of EF using 0.01 M sodium hydroxide at different temperatures cannot be studied as the drug is rapidly hydrolyzed in alkaline medium. The pH-rate profile of hydrolysis of EF in Britton–Robinson buffer solutions within the pH range 2–10 were studied.

AB - High performance liquid chromatographic (HPLC) method is presented for the determination of etofibrate (EF) and its hydrolysis products. The method was based on HPLC separation of EF from its hydrolysis products using cyanopropyl column at ambient temperature with mobile phase consisting of acetonitrile–10 mM potassium dihydrogen phosphate, pH was adjusted to 4.1 using phosphoric acid (50:50, v/v). Quantitation was achieved with UV detection at 221 nm based on peak area. The flow rate was 1.5 ml min−1. The proposed method was used to investigate the kinetics of acidic hydrolysis process of EF at different temperatures and the apparent pseudo first-order rate constant, half-life and activation energy were calculated. The kinetics of alkaline hydrolysis process of EF using 0.01 M sodium hydroxide at different temperatures cannot be studied as the drug is rapidly hydrolyzed in alkaline medium. The pH-rate profile of hydrolysis of EF in Britton–Robinson buffer solutions within the pH range 2–10 were studied.

KW - Chemistry

KW - Etofibrate

KW - HPLC

KW - Kinetics of hydrolysis

KW - pH-rate profile

UR - http://www.scopus.com/inward/record.url?scp=33845216929&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/7afe123b-142c-389e-b85c-71ce377fafd1/

U2 - 10.1016/j.jpba.2006.07.001

DO - 10.1016/j.jpba.2006.07.001

M3 - Journal articles

VL - 43

SP - 196

EP - 203

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

IS - 1

ER -