Expression of cyclooxygenase isozymes during morphogenesis and cycling of pelage hair follicles in mouse skin. Precocious Onset of the First Catagen Phase and Alopecia upon Cyclooxygenase-2 Overexpression

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet

Standard

Expression of cyclooxygenase isozymes during morphogenesis and cycling of pelage hair follicles in mouse skin. Precocious Onset of the First Catagen Phase and Alopecia upon Cyclooxygenase-2 Overexpression. / Müller-Decker, Karin; Leder, Christoph; Neumann, Melanie et al.
in: Journal of Investigative Dermatolgy , Jahrgang 121, Nr. 4, 01.10.2003, S. 661-668.

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet

Harvard

APA

Vancouver

Bibtex

@article{6939baa3e0734e7190173a6889342af0,
title = "Expression of cyclooxygenase isozymes during morphogenesis and cycling of pelage hair follicles in mouse skin.: Precocious Onset of the First Catagen Phase and Alopecia upon Cyclooxygenase-2 Overexpression",
abstract = "Cyclooxygenase (COX)-1 and -2 catalyze the key reaction in prostaglandin biosynthesis. Whereas COX-1 is found in most tissues, COX-2, with a few exceptions, is not expressed in normal tissues but becomes transiently induced in the course of inflammatory reactions. In many neoplastic epithelia, COX-2 is constitutively overexpressed. Here we show that COX isozymes are spatiotemporally expressed during morphogenesis of dorsal skin epithelium of NMRI mice. COX-1 and COX-2 mRNA and protein were detected in embryonic and postnatal epidermal tissue by RT-PCR, northern blot, and immunoblot analysis indicating that both isoforms may contribute to prostaglandin production. Being barely detectable in interfollicular epidermis and resting hair follicles of adult mice, COX-2 protein appeared in embryonic skin first in epidermal precursor cells and later on in the basal cells and the peridermal layer of the stratified epidermis. In the course of pelage hair follicle morphogenesis, COX-2 remained expressed in the basal interfollicular compartment and, in addition, became apparent in elongated hair germs and hair pegs and later on in the outer root sheath cells of the distal and proximal hair follicles as well as in basal sebaceous gland cells. During the subsequent synchronous phases of hair cycling, COX-2 expression declined in catagen, was barely detectable in telogen, and was reinduced in the basal outer root sheath and basal sebaceous gland cells of anagen hair follicles. COX-1 immunosignals were detected predominantly in the interfollicular spinous and granular layers of the developing, neonatal, and adult epidermis but not in follicular epithelial cells of developing or cycling hair follicles. Dendritic cells in the interfollicular epidermis and distal hair follicles were also COX-1-positive. Transgenic overexpression of COX-2 under the control of a keratin 5 promoter in basal cells of the interfollicular and follicular epidermis induced a precocious entry into the first catagen stage of postnatal hair follicle cycling and a subsequent disturbance of hair follicle phasing. Furthermore, transgenic mice developed an alopecia. Inhibition of transgenic COX-2 activity by feeding the specific COX-2 inhibitor valdecoxib suppressed the development of alopecia, indicating that COX-2-mediated prostaglandin synthesis is involved in hair follicle biology.",
keywords = "Chemistry, epidermis, transgenic, valdecoxib, Biology",
author = "Karin M{\"u}ller-Decker and Christoph Leder and Melanie Neumann and Gitta Neufang and Christiane Bayerl and J{\"u}rgen Schweizer and Friedrich Marks and Gerhard F{\"u}rstenberger",
year = "2003",
month = oct,
day = "1",
doi = "10.1046/j.1523-1747.2003.12473.x",
language = "English",
volume = "121",
pages = "661--668",
journal = "Journal of Investigative Dermatolgy ",
issn = "1523-1747",
publisher = "Elsevier B.V.",
number = "4",

}

RIS

TY - JOUR

T1 - Expression of cyclooxygenase isozymes during morphogenesis and cycling of pelage hair follicles in mouse skin.

T2 - Precocious Onset of the First Catagen Phase and Alopecia upon Cyclooxygenase-2 Overexpression

AU - Müller-Decker, Karin

AU - Leder, Christoph

AU - Neumann, Melanie

AU - Neufang, Gitta

AU - Bayerl, Christiane

AU - Schweizer, Jürgen

AU - Marks, Friedrich

AU - Fürstenberger, Gerhard

PY - 2003/10/1

Y1 - 2003/10/1

N2 - Cyclooxygenase (COX)-1 and -2 catalyze the key reaction in prostaglandin biosynthesis. Whereas COX-1 is found in most tissues, COX-2, with a few exceptions, is not expressed in normal tissues but becomes transiently induced in the course of inflammatory reactions. In many neoplastic epithelia, COX-2 is constitutively overexpressed. Here we show that COX isozymes are spatiotemporally expressed during morphogenesis of dorsal skin epithelium of NMRI mice. COX-1 and COX-2 mRNA and protein were detected in embryonic and postnatal epidermal tissue by RT-PCR, northern blot, and immunoblot analysis indicating that both isoforms may contribute to prostaglandin production. Being barely detectable in interfollicular epidermis and resting hair follicles of adult mice, COX-2 protein appeared in embryonic skin first in epidermal precursor cells and later on in the basal cells and the peridermal layer of the stratified epidermis. In the course of pelage hair follicle morphogenesis, COX-2 remained expressed in the basal interfollicular compartment and, in addition, became apparent in elongated hair germs and hair pegs and later on in the outer root sheath cells of the distal and proximal hair follicles as well as in basal sebaceous gland cells. During the subsequent synchronous phases of hair cycling, COX-2 expression declined in catagen, was barely detectable in telogen, and was reinduced in the basal outer root sheath and basal sebaceous gland cells of anagen hair follicles. COX-1 immunosignals were detected predominantly in the interfollicular spinous and granular layers of the developing, neonatal, and adult epidermis but not in follicular epithelial cells of developing or cycling hair follicles. Dendritic cells in the interfollicular epidermis and distal hair follicles were also COX-1-positive. Transgenic overexpression of COX-2 under the control of a keratin 5 promoter in basal cells of the interfollicular and follicular epidermis induced a precocious entry into the first catagen stage of postnatal hair follicle cycling and a subsequent disturbance of hair follicle phasing. Furthermore, transgenic mice developed an alopecia. Inhibition of transgenic COX-2 activity by feeding the specific COX-2 inhibitor valdecoxib suppressed the development of alopecia, indicating that COX-2-mediated prostaglandin synthesis is involved in hair follicle biology.

AB - Cyclooxygenase (COX)-1 and -2 catalyze the key reaction in prostaglandin biosynthesis. Whereas COX-1 is found in most tissues, COX-2, with a few exceptions, is not expressed in normal tissues but becomes transiently induced in the course of inflammatory reactions. In many neoplastic epithelia, COX-2 is constitutively overexpressed. Here we show that COX isozymes are spatiotemporally expressed during morphogenesis of dorsal skin epithelium of NMRI mice. COX-1 and COX-2 mRNA and protein were detected in embryonic and postnatal epidermal tissue by RT-PCR, northern blot, and immunoblot analysis indicating that both isoforms may contribute to prostaglandin production. Being barely detectable in interfollicular epidermis and resting hair follicles of adult mice, COX-2 protein appeared in embryonic skin first in epidermal precursor cells and later on in the basal cells and the peridermal layer of the stratified epidermis. In the course of pelage hair follicle morphogenesis, COX-2 remained expressed in the basal interfollicular compartment and, in addition, became apparent in elongated hair germs and hair pegs and later on in the outer root sheath cells of the distal and proximal hair follicles as well as in basal sebaceous gland cells. During the subsequent synchronous phases of hair cycling, COX-2 expression declined in catagen, was barely detectable in telogen, and was reinduced in the basal outer root sheath and basal sebaceous gland cells of anagen hair follicles. COX-1 immunosignals were detected predominantly in the interfollicular spinous and granular layers of the developing, neonatal, and adult epidermis but not in follicular epithelial cells of developing or cycling hair follicles. Dendritic cells in the interfollicular epidermis and distal hair follicles were also COX-1-positive. Transgenic overexpression of COX-2 under the control of a keratin 5 promoter in basal cells of the interfollicular and follicular epidermis induced a precocious entry into the first catagen stage of postnatal hair follicle cycling and a subsequent disturbance of hair follicle phasing. Furthermore, transgenic mice developed an alopecia. Inhibition of transgenic COX-2 activity by feeding the specific COX-2 inhibitor valdecoxib suppressed the development of alopecia, indicating that COX-2-mediated prostaglandin synthesis is involved in hair follicle biology.

KW - Chemistry

KW - epidermis

KW - transgenic

KW - valdecoxib

KW - Biology

UR - http://www.scopus.com/inward/record.url?scp=0141887557&partnerID=8YFLogxK

U2 - 10.1046/j.1523-1747.2003.12473.x

DO - 10.1046/j.1523-1747.2003.12473.x

M3 - Journal articles

VL - 121

SP - 661

EP - 668

JO - Journal of Investigative Dermatolgy

JF - Journal of Investigative Dermatolgy

SN - 1523-1747

IS - 4

ER -

DOI

Zuletzt angesehen

Publikationen

  1. Selbstgesteuertes Lernen mit Neuen Medien im Fremdsprachenunterricht - Eine Bestandsaufnahme
  2. Die heiligen drei Narren
  3. Dehnen und Beweglichkeitstraining
  4. Musik & Empowerment
  5. Die Unternehmensstiftung: unter wirtschaftsrechtlichen Aspekten eine Alternative der Unternehmensnachfolge?
  6. Elementgehalte von Kiefern- und Fichtennadeln südlich von Frankfurt/Main
  7. Bilanzrechtsmodernisierung: Wandel von einer „offenen“ zu einer „stillen“ Bilanzpolitik
  8. Teacher’s ethnic teasing
  9. Chaos-Forschung und ihre Perspektiven für die Psychologie
  10. Tourismus ohne Raum
  11. Quo vadis ErzieherInnenausbildung? Akademische Studiengänge in der Elementarpädagogik
  12. Repatriation outcomes affecting corporate ROI
  13. Mathematik für alle
  14. Integrative Entrepreneurshipforschung - Identifikation von Schnittstellen zwischen soziologischer und ökonomischer Perspektive
  15. The tiger beetles (Coleoptera, Cicindelidae) of the southern Levant and adjacent territories
  16. Los medios de comunicación en Europa
  17. Front-Räume. Ernst Jüngers „In Stahlgewittern“ und die Figur des „Feindes“
  18. Ökologisierung von Finanzmärkten
  19. Entschädigungslose Enteignung russisch kontrollierter Energieinfrastruktur
  20. Climate change and modelling of extreme temperatures in Switzerland
  21. Satellite Earth Observations and Their Impact on Society and Policy, (eds Masami Onoda & Oran R. Young). 1st ed. Berlin: SpringerOpen. 2017
  22. Lernaufgaben nachhaltig gedacht
  23. Verändertes Lernen - verbesserte Leistungen? Zur Entwicklung von Schülerfähigkeiten bei SINUS-Transfer
  24. Anni Albers’s ‘Pliable Plane’
  25. (Gegen-)Öffentlichkeiten im 21. Jahrhundert