Elution of monomers from three different bonding systems and their antibacterial effect

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet

Standard

Elution of monomers from three different bonding systems and their antibacterial effect. / Polydorou, Olga; Rogatti, Philipp; Bolek, Richard; Wolkewitz, Martin; Kümmerer, Klaus; Hellwig, Elmar.

in: Odontology / the Society of the Nippon Dental University, Jahrgang 101, Nr. 2, 07.2013, S. 170-176.

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet

Harvard

APA

Vancouver

Bibtex

@article{a213379e761b4697b37d0e636824a426,
title = "Elution of monomers from three different bonding systems and their antibacterial effect",
abstract = "The aim of the present study was to evaluate the release of monomers from three bonding systems and to correlate it with their antibacterial effect. Three bonding systems (Optibond FL{\textregistered}, Xeno III{\textregistered} and Clearfil{\texttrademark} Protect Bond) were tested after storage in ethanol 75 % and human saliva. Twenty samples (n = 10/medium) of each bonding material were prepared and polymerized according to the manufacturers' instructions. Each sample was stored in 1 ml of the respective storage medium. The medium was renewed after 24 h, 7 days, and 28 days and was analysed by LC-MS/MS for the release of substances. Additionally, the antibacterial effect of the unpolymerized components of each bonding system and their polymerized mixture was tested using agar disc-diffusion test with Streptococcus mutans. Only HEMA was found to be released. The amount of HEMA detected in the ethanol samples was significantly higher compared to the saliva samples (p < 0.0001). The release of HEMA was as follows: Clearfil{\texttrademark} Protect Bond < Optibond FL{\textregistered} < Xeno III {\textregistered}. According to the agar disc-diffusion test, all materials exhibited certain antibacterial activity. The release of HEMA from all tested materials even after storing in human saliva increases the concerns about their toxicity. Their antibacterial effect seems not be due to the release of substances.",
keywords = "Chemistry, Bonding systems, HEMA, HPLC, Antibacterial activity, Sustainability Science",
author = "Olga Polydorou and Philipp Rogatti and Richard Bolek and Martin Wolkewitz and Klaus K{\"u}mmerer and Elmar Hellwig",
note = "Copyright 2012 Elsevier B.V., All rights reserved.",
year = "2013",
month = jul,
doi = "10.1007/s10266-012-0071-4",
language = "English",
volume = "101",
pages = "170--176",
journal = "Odontology / the Society of the Nippon Dental University",
issn = "1618-1247",
publisher = "Springer Japan",
number = "2",

}

RIS

TY - JOUR

T1 - Elution of monomers from three different bonding systems and their antibacterial effect

AU - Polydorou, Olga

AU - Rogatti, Philipp

AU - Bolek, Richard

AU - Wolkewitz, Martin

AU - Kümmerer, Klaus

AU - Hellwig, Elmar

N1 - Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2013/7

Y1 - 2013/7

N2 - The aim of the present study was to evaluate the release of monomers from three bonding systems and to correlate it with their antibacterial effect. Three bonding systems (Optibond FL®, Xeno III® and Clearfil™ Protect Bond) were tested after storage in ethanol 75 % and human saliva. Twenty samples (n = 10/medium) of each bonding material were prepared and polymerized according to the manufacturers' instructions. Each sample was stored in 1 ml of the respective storage medium. The medium was renewed after 24 h, 7 days, and 28 days and was analysed by LC-MS/MS for the release of substances. Additionally, the antibacterial effect of the unpolymerized components of each bonding system and their polymerized mixture was tested using agar disc-diffusion test with Streptococcus mutans. Only HEMA was found to be released. The amount of HEMA detected in the ethanol samples was significantly higher compared to the saliva samples (p < 0.0001). The release of HEMA was as follows: Clearfil™ Protect Bond < Optibond FL® < Xeno III ®. According to the agar disc-diffusion test, all materials exhibited certain antibacterial activity. The release of HEMA from all tested materials even after storing in human saliva increases the concerns about their toxicity. Their antibacterial effect seems not be due to the release of substances.

AB - The aim of the present study was to evaluate the release of monomers from three bonding systems and to correlate it with their antibacterial effect. Three bonding systems (Optibond FL®, Xeno III® and Clearfil™ Protect Bond) were tested after storage in ethanol 75 % and human saliva. Twenty samples (n = 10/medium) of each bonding material were prepared and polymerized according to the manufacturers' instructions. Each sample was stored in 1 ml of the respective storage medium. The medium was renewed after 24 h, 7 days, and 28 days and was analysed by LC-MS/MS for the release of substances. Additionally, the antibacterial effect of the unpolymerized components of each bonding system and their polymerized mixture was tested using agar disc-diffusion test with Streptococcus mutans. Only HEMA was found to be released. The amount of HEMA detected in the ethanol samples was significantly higher compared to the saliva samples (p < 0.0001). The release of HEMA was as follows: Clearfil™ Protect Bond < Optibond FL® < Xeno III ®. According to the agar disc-diffusion test, all materials exhibited certain antibacterial activity. The release of HEMA from all tested materials even after storing in human saliva increases the concerns about their toxicity. Their antibacterial effect seems not be due to the release of substances.

KW - Chemistry

KW - Bonding systems

KW - HEMA

KW - HPLC

KW - Antibacterial activity

KW - Sustainability Science

UR - http://www.scopus.com/inward/record.url?scp=84881034050&partnerID=8YFLogxK

U2 - 10.1007/s10266-012-0071-4

DO - 10.1007/s10266-012-0071-4

M3 - Journal articles

C2 - 22684585

VL - 101

SP - 170

EP - 176

JO - Odontology / the Society of the Nippon Dental University

JF - Odontology / the Society of the Nippon Dental University

SN - 1618-1247

IS - 2

ER -

DOI