TY - JOUR

T1 - Elevated temperature differently affects foliar nitrogen partitioning in seedlings of diverse Douglas fir provenances

AU - Du, Baoguo

AU - Jansen, Kirstin

AU - Junker, Laura Verena

AU - Eiblmeier, Monika

AU - Kreuzwieser, Jürgen

AU - Gessler, Arthur

AU - Ensminger, Ingo

AU - Rennenberg, Heinz

PY - 2014/10/1

Y1 - 2014/10/1

N2 - Global climate change causes an increase in ambient air temperature, a major environmental factor influencing plant physiology and growth that already has been perceived at the regional scale and is expected to become even more severe in the future. In the present study, we investigated the effect of elevated ambient air temperature on the nitrogen metabolism of two interior provenances of Douglas fir (Pseudotsuga menziesii var. glauca) originating from contrasting habitats, namely the provenances Monte Creek (MC) from a drier environment and Pend Oreille (PO) from a more humid environment. Three- to four-year-old seedlings of the two provenances were grown for 3 months in controlled environments under either control temperature (day 20°C, night 15°C) or high temperature (HT, 30/25°C) conditions. Total nitrogen (N), soluble protein, chlorophyll and total amino acid (TAA) contents as well as individual amino acid concentrations were determined in both current-year and previous-year needles. Our results show that the foliar total N contents of the two provenances were unaffected by HT. Arginine, lysine, proline, glutamate and glutamine were the most abundant amino acids, which together contributed ∼88% to the TAA pool of current- and previous-year needles. High temperature decreased the contents of most amino acids of the glutamate family (i.e., arginine, proline, ornithine and glutamine) in current-year needles. However, HT did not affect the concentrations of metabolites related to the photorespiratory pathway, such as NH 4 +, glycine and serine. In general, current-year needles were considerably more sensitive to HT than previous-year needles. Moreover, provenance PO originating from a mesic environment showed stronger responses to HT than provenance MC. Our results indicate provenance-specific plasticity in the response of Douglas fir to growth temperature. Provenance-specific effects of elevated temperature on N-use efficiency suggest that origin might determine the sensitivity and growth potential of Douglas fir trees in a future warmer climate.

AB - Global climate change causes an increase in ambient air temperature, a major environmental factor influencing plant physiology and growth that already has been perceived at the regional scale and is expected to become even more severe in the future. In the present study, we investigated the effect of elevated ambient air temperature on the nitrogen metabolism of two interior provenances of Douglas fir (Pseudotsuga menziesii var. glauca) originating from contrasting habitats, namely the provenances Monte Creek (MC) from a drier environment and Pend Oreille (PO) from a more humid environment. Three- to four-year-old seedlings of the two provenances were grown for 3 months in controlled environments under either control temperature (day 20°C, night 15°C) or high temperature (HT, 30/25°C) conditions. Total nitrogen (N), soluble protein, chlorophyll and total amino acid (TAA) contents as well as individual amino acid concentrations were determined in both current-year and previous-year needles. Our results show that the foliar total N contents of the two provenances were unaffected by HT. Arginine, lysine, proline, glutamate and glutamine were the most abundant amino acids, which together contributed ∼88% to the TAA pool of current- and previous-year needles. High temperature decreased the contents of most amino acids of the glutamate family (i.e., arginine, proline, ornithine and glutamine) in current-year needles. However, HT did not affect the concentrations of metabolites related to the photorespiratory pathway, such as NH 4 +, glycine and serine. In general, current-year needles were considerably more sensitive to HT than previous-year needles. Moreover, provenance PO originating from a mesic environment showed stronger responses to HT than provenance MC. Our results indicate provenance-specific plasticity in the response of Douglas fir to growth temperature. Provenance-specific effects of elevated temperature on N-use efficiency suggest that origin might determine the sensitivity and growth potential of Douglas fir trees in a future warmer climate.

KW - Sustainability Science

KW - Amino acid

KW - Arginine

KW - Pseudotsuga menziesii

UR - http://www.scopus.com/inward/record.url?scp=84923516657&partnerID=8YFLogxK

U2 - 10.1093/treephys/tpu074

DO - 10.1093/treephys/tpu074

M3 - Journal articles

C2 - 25240727

VL - 34

SP - 1090

EP - 1101

JO - Tree Physiology

JF - Tree Physiology

SN - 0829-318X

IS - 10

ER -