Element distribution in the corrosion layer and cytotoxicity of alloy Mg-10Dy during in vitro biodegradation
Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet
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Element distribution in the corrosion layer and cytotoxicity of alloy Mg-10Dy during in vitro biodegradation. / Yang, Lei; Hort, Norbert; Laipple, Daniel et al.
in: Acta Biomaterialia, Jahrgang 9, Nr. 10, 11.2013, S. 8475-8487.Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet
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TY - JOUR
T1 - Element distribution in the corrosion layer and cytotoxicity of alloy Mg-10Dy during in vitro biodegradation
AU - Yang, Lei
AU - Hort, Norbert
AU - Laipple, Daniel
AU - Höche, Daniel
AU - Huang, Yuanding
AU - Kainer, Karl Ulrich
AU - Willumeit, Regine
AU - Feyerabend, Frank
PY - 2013/11
Y1 - 2013/11
N2 - The present work investigates the corrosion behaviour, the element distribution in the corrosion layer and the cytocompatibility of alloy Mg-10Dy. The corrosion experiments were performed in a cell culture medium (CCM) under cell culture conditions close to the in vivo environment. The element distribution on the surface as well as in cross-sections of the corrosion layer was investigated using scanning electron microscopy, energy-dispersive X-ray analysis, X-ray photoelectron spectroscopy and X-ray diffraction. The cytocompatibility of alloy Mg-10Dy with primary human osteoblasts was evaluated by MTT, cell adhesion and live/dead staining tests. The results show that the corrosion layer was enriched in Dy, while the P and Ca content gradually decreased from the surface to the bottom of the corrosion layer. In addition, large amounts of MgCO3·3H2O formed in the corrosion layer after 28 days immersion. Both extracts and the Dy-enriched corrosion layer of alloy Mg-10Dy showed no cytotoxicity to primary human osteoblasts.
AB - The present work investigates the corrosion behaviour, the element distribution in the corrosion layer and the cytocompatibility of alloy Mg-10Dy. The corrosion experiments were performed in a cell culture medium (CCM) under cell culture conditions close to the in vivo environment. The element distribution on the surface as well as in cross-sections of the corrosion layer was investigated using scanning electron microscopy, energy-dispersive X-ray analysis, X-ray photoelectron spectroscopy and X-ray diffraction. The cytocompatibility of alloy Mg-10Dy with primary human osteoblasts was evaluated by MTT, cell adhesion and live/dead staining tests. The results show that the corrosion layer was enriched in Dy, while the P and Ca content gradually decreased from the surface to the bottom of the corrosion layer. In addition, large amounts of MgCO3·3H2O formed in the corrosion layer after 28 days immersion. Both extracts and the Dy-enriched corrosion layer of alloy Mg-10Dy showed no cytotoxicity to primary human osteoblasts.
KW - Cytocompatibility
KW - Element distribution
KW - In vitro corrosion
KW - Mg-10Dy alloy
KW - Engineering
UR - http://www.scopus.com/inward/record.url?scp=84884675978&partnerID=8YFLogxK
U2 - 10.1016/j.actbio.2012.10.001
DO - 10.1016/j.actbio.2012.10.001
M3 - Journal articles
C2 - 23063554
AN - SCOPUS:84884675978
VL - 9
SP - 8475
EP - 8487
JO - Acta Biomaterialia
JF - Acta Biomaterialia
SN - 1742-7061
IS - 10
ER -