Development and validation of chemometrics-assisted spectrophotometry and liquid chromatography methods for the simultaneous determination of the active ingredients in two multicomponent mixtures containing chlorpheniramine maleate and phenylpropanolamine hydrochloride.

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet

Authors

  • Ghada M. Hadad
  • Alaa El-Gindy
  • Waleed M. M. Mahmoud

Multivariate spectrophotometric calibration and liquid chromatography (LC) methods were used for the simultaneous determination of the active ingredients in 2 multicomponent mixtures containing chlorpheniramine maleate and phenylpropanolamine hydrochloride with ibuprofen and caffeine (mixture 1) or with propyphenazone (mixture 2). For the multivariate spectrophotometric calibration methods, principal component regression (PCR) and partial least squares (PLS-1), a calibration set of the mixtures consisting of the components of each mixture was prepared in distilled water. A leave-1-out cross-validation procedure was used to find the optimum numbers of latent variables. Analytical parameters such as sensitivity, selectivity, analytical sensitivity, limit of quantitation, and limit of detection were determined for both PLS-1 and PCR. The LC method depends on the use of a cyanopropyl column with the mobile phase acetonitriie-12 mM ammonium acetate, pH 5.0 (25 + 75, v/v), for mixture 1 or acetonitrile-10 mM potassium dihydrogen phosphate, pH 4.7 (45 + 55, v/v), for mixture 2; the UV detector was set at 212 nm. In spite of the presence of a high degree of spectral overlap of these components, they were rapidly and simultaneously determined with high accuracy and precision, with no interference from the matrix excipients. The proposed methods were successfully applied to the analysis of pharmaceutical formulations and laboratory-prepared mixtures containing the 2 multicomponent combinations.

OriginalspracheEnglisch
ZeitschriftJournal of AOAC International
Jahrgang90
Ausgabenummer4
Seiten (von - bis)957-970
Anzahl der Seiten14
ISSN1060-3271
DOIs
PublikationsstatusErschienen - 01.07.2007
Extern publiziertJa

DOI

Zuletzt angesehen

Forschende

  1. Pierangelo Maset

Publikationen

  1. Media Review: Extrapolations - A View from OS4F
  2. Identification of Parameters and States in PMSMs
  3. A cross-scale assessment of productivity–diversity relationships
  4. Impact assessment of emissions stabilization scenarios with and without induced technological change
  5. Process Analyses of Grounding in Chat-based CSCL
  6. Export Boosting Policies and Firm Performance
  7. Can Geodesign Be Used to Facilitate Boundary Management for Planning and Implementation of Nature-based Solutions?
  8. New developments in space technology
  9. Conception and analysis of Cascaded Dual Kalman Filters as virtual sensors for mastication activity of stomatognathic craniomandibular system
  10. Wie lang sollte eine Rollstuhlrampe sein?
  11. Heterogenitätssensible Hochschullehre
  12. Who’s afraid of the senses? Organization, management and the return of the sensorium
  13. A transdisciplinary evaluation framework for the assessment of integration in boundary-crossing collaborations in teacher education
  14. Robust Control as a Mathematical Paradigm for Innovative Engineering Applications
  15. Calculation of Physicochemical Properties for Short- and Medium-Chain Chlorinated Paraffins
  16. Hommage to the unknown viewers
  17. An indirectly controlled high-speed servo valve for IC engines using piezo actuators
  18. Me and AAC - Alternative and Augmented Communication in West Germany from a Biographical and Media Archaeological Perspective
  19. Does board composition have an impact on CSR reporting?
  20. Approaches and Lessons in Political Career Research
  21. Benchmarking question answering systems
  22. Mobilität
  23. Navigating in the Digital Jungle: Articulating Combinatory Affordances of Digital Infrastructures for Collaboration
  24. Design for the triple topline
  25. X Machina and the World of Tomorrow