An Advanced Double Column-Switching Technique (LC-LC) for Liquid Chromatography/Electrospray Ionisation Tandem Mass Spectrometry for Fully Automated Analysis of Caspofungin

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet

Authors

Caspofungin (MK-0991; L-743,872) is the first representative of a new important class of antifungal agents, the glucan synthesis inhibitors. To the authors' best knowledge, to date only one high-performance liquid chromatography (HPLC) method has been published for the determination of caspofungin in serum. Severe difficulties with sorption were described. We developed a new method which addresses these difficulties using an advanced column-switching technique for fully automated analysis of caspofungin in serum without any pre-treatment. Extraction was performed automatically inline, using a diol column, followed by chromatography on a CN column. Detection was performed by electrospray ionisation tandem mass spectrometry (ESI-MS/MS) with isolation and fragmentation in the positive ion mode. Total analysis time was 30 min. Detection of caspofungin was achieved by retention time, isolation and fragmentation of the double positively charged caspofungin ion. This LC/MS assay was validated for between-run accuracy (max. 110%) and precision (max. CV 16.1%). The lower limit of quantification was 0.2 μg/mL. The analytical method with fully automated inline extraction of caspofungin described here removes the need for difficult and time-consuming sample pre-treatment. Sorption of caspofungin is not of importance. Additional advantages of the new method are that only a small quantity of serum (5 μL) is needed and that the method is very specific. Copyright © 2004 John Wiley & Sons, Ltd.
OriginalspracheEnglisch
ZeitschriftRapid Communications in Mass Spectrometry
Jahrgang18
Ausgabenummer23
Seiten (von - bis)2871-2877
Anzahl der Seiten7
DOIs
PublikationsstatusErschienen - 01.11.2004
Extern publiziertJa

    Fachgebiete

  • accuracy, AGENTS, ANALYSIS, antifungal, ANTIFUNGAL AGENT, ASSAY, Caspofungin, chromatography, COLUMN, column switching, column-switching, detection, determination, Electrospray, EXTRACTION, GERMANY, HIGH PERFORMANCE LIQUID CHROMATOGRAPHY, HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY, HPLC, human plasma, INHIBITOR, INHIBITORS, Ionisation, KNOWLEDGE, LC, MS, liquid, liquid chromatography, LIQUID-CHROMATOGRAPHY, mass spectrometry, MASS-SPECTROMETRY, Method, MK-0991, MODE, pharmacokinetics, PRETREATMENT, QUANTIFICATION, RABBITS, retention, Serum, Sorption, SPECTROMETRY, tandem mass spectrometry, URINE
  • Chemie

DOI

Zuletzt angesehen

Publikationen

  1. Communicating effectively about CSR on Twitter
  2. Von "cool" zu Klärung
  3. National ecosystem restoration pledges are mismatched with social-ecological enabling conditions
  4. Morphometric differentiation in a specialised snail predatior
  5. Cultivating dispersed collectivity
  6. Analysis of mechanical properties and microstructure of single and double-pass friction stir welded T-joints for aluminium stiffened Panels
  7. Demographic change in work organisations
  8. Soziale Netzwerke im Internet
  9. TACKLING THE GLOBAL WASTE PROBLEM AS A MULTI-LEVEL PROCESS
  10. Understanding Resulting Interdependencies Within Production Planning And Control In Hybrid Manufacturing/Remanufacturing Systems
  11. The Achilles' Heel of Absolute Power
  12. CSR and tax avoidance: A review of empirical research
  13. Markenwert
  14. Messung von Markenvorstellungen
  15. Making sense of sustainability transitions locally
  16. Start-up Consulting in the German Language Realm
  17. Der Sandbox Innovation Process: Wie Vielfalt in Open-Innovation-Communities genutzt werden
  18. Temperature-dependent mechanical behavior of aluminum AM structures generated via multi-layer friction surfacing
  19. On entrepreneurial risk-taking and the macroeconomic effects of financial constraints
  20. Earnings less risk-free interest charge (ERIC) and stock returns: ERIC’s relative and incremental information content in a European sample
  21. Schwerbehinderung
  22. What explains the performance of participatory governance?
  23. Kommunikative Interferenzen
  24. Article 72 CISG
  25. DESI
  26. Feedback mit eigenen Augen
  27. An analysis of the requirements for DSS on integrated river basin management
  28. Grounding Space