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A suite of multiplexed microsatellite loci for the ground beetle Abax parallelepipedus (Piller and Mitterpacher, 1783) (Coleoptera, Carabidae)

Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet

Standard

A suite of multiplexed microsatellite loci for the ground beetle Abax parallelepipedus (Piller and Mitterpacher, 1783) (Coleoptera, Carabidae). / Marcus, Tamar; Assmann, Thorsten; Durka, Walter et al.
in: Conservation Genetics Resources, Jahrgang 5, Nr. 4, 12.2013, S. 1151-1156.

Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet

Harvard

Marcus, T, Assmann, T, Durka, W & Drees, C 2013, 'A suite of multiplexed microsatellite loci for the ground beetle Abax parallelepipedus (Piller and Mitterpacher, 1783) (Coleoptera, Carabidae)', Conservation Genetics Resources, Jg. 5, Nr. 4, S. 1151-1156. https://doi.org/10.1007/s12686-013-9985-6

APA

Marcus, T., Assmann, T., Durka, W., & Drees, C. (2013). A suite of multiplexed microsatellite loci for the ground beetle Abax parallelepipedus (Piller and Mitterpacher, 1783) (Coleoptera, Carabidae). Conservation Genetics Resources, 5(4), 1151-1156. https://doi.org/10.1007/s12686-013-9985-6

Vancouver

Marcus T, Assmann T, Durka W, Drees C. A suite of multiplexed microsatellite loci for the ground beetle Abax parallelepipedus (Piller and Mitterpacher, 1783) (Coleoptera, Carabidae). Conservation Genetics Resources. 2013 Dez;5(4):1151-1156. doi: 10.1007/s12686-013-9985-6

Bibtex

@article{2ea0303c7ba54176bf41df6677aa36d1,
title = "A suite of multiplexed microsatellite loci for the ground beetle Abax parallelepipedus (Piller and Mitterpacher, 1783) (Coleoptera, Carabidae)",
abstract = "We report two sets of polymorphic, multiplexed microsatellite markers for the ground beetle Abax parallelepipedus. As the species is flightless, restricted to forests and affected by habitat fragmentation it can serve as a model species for landscape and conservation genetics. A complete set of 20 loci can be amplified in five PCR reactions and sequenced in two rounds, and a subset of 14 loci can be analyzed together in one PCR run and one sequencing round. In a scan of 3,432 individuals from across Germany using the 14 loci subset, we found between three and 14 alleles per locus. After accounting for two loci that are apparently sex-linked, no significant deviations from Hardy-Weinberg equilibrium were found. None of the loci showed evidence for the presence of null alleles. No overall linkage disequilibrium was detected. Some of the loci can also be used to study other Abax species.",
keywords = "Ecosystems Research, Abax parallelepipedus, Carabidae, Landscape genetics, Primers, Biology",
author = "Tamar Marcus and Thorsten Assmann and Walter Durka and Claudia Drees",
year = "2013",
month = dec,
doi = "10.1007/s12686-013-9985-6",
language = "English",
volume = "5",
pages = "1151--1156",
journal = "Conservation Genetics Resources",
issn = "1877-7252",
publisher = "Springer Science and Business Media B.V.",
number = "4",

}

RIS

TY - JOUR

T1 - A suite of multiplexed microsatellite loci for the ground beetle Abax parallelepipedus (Piller and Mitterpacher, 1783) (Coleoptera, Carabidae)

AU - Marcus, Tamar

AU - Assmann, Thorsten

AU - Durka, Walter

AU - Drees, Claudia

PY - 2013/12

Y1 - 2013/12

N2 - We report two sets of polymorphic, multiplexed microsatellite markers for the ground beetle Abax parallelepipedus. As the species is flightless, restricted to forests and affected by habitat fragmentation it can serve as a model species for landscape and conservation genetics. A complete set of 20 loci can be amplified in five PCR reactions and sequenced in two rounds, and a subset of 14 loci can be analyzed together in one PCR run and one sequencing round. In a scan of 3,432 individuals from across Germany using the 14 loci subset, we found between three and 14 alleles per locus. After accounting for two loci that are apparently sex-linked, no significant deviations from Hardy-Weinberg equilibrium were found. None of the loci showed evidence for the presence of null alleles. No overall linkage disequilibrium was detected. Some of the loci can also be used to study other Abax species.

AB - We report two sets of polymorphic, multiplexed microsatellite markers for the ground beetle Abax parallelepipedus. As the species is flightless, restricted to forests and affected by habitat fragmentation it can serve as a model species for landscape and conservation genetics. A complete set of 20 loci can be amplified in five PCR reactions and sequenced in two rounds, and a subset of 14 loci can be analyzed together in one PCR run and one sequencing round. In a scan of 3,432 individuals from across Germany using the 14 loci subset, we found between three and 14 alleles per locus. After accounting for two loci that are apparently sex-linked, no significant deviations from Hardy-Weinberg equilibrium were found. None of the loci showed evidence for the presence of null alleles. No overall linkage disequilibrium was detected. Some of the loci can also be used to study other Abax species.

KW - Ecosystems Research

KW - Abax parallelepipedus

KW - Carabidae

KW - Landscape genetics

KW - Primers

KW - Biology

UR - http://www.scopus.com/inward/record.url?scp=84892899538&partnerID=8YFLogxK

U2 - 10.1007/s12686-013-9985-6

DO - 10.1007/s12686-013-9985-6

M3 - Journal articles

AN - SCOPUS:84892899538

VL - 5

SP - 1151

EP - 1156

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

SN - 1877-7252

IS - 4

ER -

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