A microsystem for growth inhibition test of Enterococcus faecalis based on impedance measurement
Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet
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in: Sensors and Actuators B: Chemical, Jahrgang 118, Nr. 1-2, 25.10.2006, S. 182-191.
Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet
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TY - JOUR
T1 - A microsystem for growth inhibition test of Enterococcus faecalis based on impedance measurement
AU - Spiller, E.
AU - Schöll, A.
AU - Alexy, R.
AU - Kümmerer, K.
AU - Urban , A. G.
PY - 2006/10/25
Y1 - 2006/10/25
N2 - Bacterial tests such as growth inhibition tests are the only convenient investigation method to detect the impacts of toxic water soluble materials, e.g. antibiotics, on bacteria. The principle of these inhibition tests is the detection of cell numbers by measuring the optical density. With this time consuming cumbersome and complicate method only endpoint detection and no monitoring of cell growth is possible. In this paper we present a conductivity measurement system based on the theory of Maxwell-Fricke. The intention of this work was to reduce the sample volume and have a standard screening platform, therefore we choose the 1536 microtiter plate format without a bottom foil as screening system for the growth inhibition test. The equivalent circuit models of the electrode-electrolyte interface and their influence to biological species were discussed. The presented sensor system in this paper can distinguish living and death cells during the continuous biomass monitoring. © 2006 Elsevier B.V. All rights reserved.
AB - Bacterial tests such as growth inhibition tests are the only convenient investigation method to detect the impacts of toxic water soluble materials, e.g. antibiotics, on bacteria. The principle of these inhibition tests is the detection of cell numbers by measuring the optical density. With this time consuming cumbersome and complicate method only endpoint detection and no monitoring of cell growth is possible. In this paper we present a conductivity measurement system based on the theory of Maxwell-Fricke. The intention of this work was to reduce the sample volume and have a standard screening platform, therefore we choose the 1536 microtiter plate format without a bottom foil as screening system for the growth inhibition test. The equivalent circuit models of the electrode-electrolyte interface and their influence to biological species were discussed. The presented sensor system in this paper can distinguish living and death cells during the continuous biomass monitoring. © 2006 Elsevier B.V. All rights reserved.
KW - Bioimpedance
KW - Antibiotic testing
KW - Conductivity measurement
KW - Enterococcus faecalis
KW - Chemistry
KW - Bioimpedance
KW - Antibiotic testing
KW - Conductivity measurement
KW - Enterococcus faecalis
UR - http://www.scopus.com/inward/record.url?scp=33747840949&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/da826260-a8d2-3778-8cf0-386538de2048/
U2 - 10.1016/j.snb.2006.04.016
DO - 10.1016/j.snb.2006.04.016
M3 - Journal articles
VL - 118
SP - 182
EP - 191
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
SN - 0925-4005
IS - 1-2
ER -