TY - JOUR

T1 - A comprehensive method for determination of fatty acids in the initial oral biofilm (pellicle)

AU - Reich, Marco

AU - Hannig, Christian

AU - Al-Ahmad, Ali

AU - Bolek, Richard

AU - Kuemmerer, Klaus

N1 - Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2012/10/1

Y1 - 2012/10/1

N2 - The acquired pellicle is a tenacious organic layer covering the surface of teeth, protecting the underlying dental hard tissues. Lipids account for about one quarter of the pellicle's dry weight and are assumed to be of considerable importance for their protective properties. Nevertheless, only preliminary information is available about the nature of lipids in the pellicle. Gas chromatography coupled with electron impact ionization mass spectrometry was used to establish a convenient analytical protocol in order to obtain a qualitative and quantitative characterization of a wide range of FAs (C 12-C 22). In situ biofilm formation was performed on bovine enamel slabs mounted on individual splints carried by 10 subjects. A modified Folch extraction procedure was adopted to extract the lipids from the detached pellicle, followed by transesterification to fatty acid methyl esters using methanol and concentrated hydrochloric acid. Tridecanoic and nonadecanoic acid were used as internal standards suitable and reliable for robust, precise and accurate measurements. The present study demonstrates, for the first time, a procedure based on a combination of innovative specimen generation and convenient sample preparation with sensitive GC-MS analysis for the determination of the fatty acid profile of the initial oral biofilm.

AB - The acquired pellicle is a tenacious organic layer covering the surface of teeth, protecting the underlying dental hard tissues. Lipids account for about one quarter of the pellicle's dry weight and are assumed to be of considerable importance for their protective properties. Nevertheless, only preliminary information is available about the nature of lipids in the pellicle. Gas chromatography coupled with electron impact ionization mass spectrometry was used to establish a convenient analytical protocol in order to obtain a qualitative and quantitative characterization of a wide range of FAs (C 12-C 22). In situ biofilm formation was performed on bovine enamel slabs mounted on individual splints carried by 10 subjects. A modified Folch extraction procedure was adopted to extract the lipids from the detached pellicle, followed by transesterification to fatty acid methyl esters using methanol and concentrated hydrochloric acid. Tridecanoic and nonadecanoic acid were used as internal standards suitable and reliable for robust, precise and accurate measurements. The present study demonstrates, for the first time, a procedure based on a combination of innovative specimen generation and convenient sample preparation with sensitive GC-MS analysis for the determination of the fatty acid profile of the initial oral biofilm.

KW - Chemistry

KW - fatty acid

KW - clinical trials

KW - derivatization

KW - saliva

KW - mass spectrometry

KW - in situ

KW - Clinical trials

KW - derivatization

KW - fatty acid

KW - in situ

KW - Mass Spectrometry

KW - saliva

KW - Sustainability Science

UR - http://www.scopus.com/inward/record.url?scp=84866180250&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/4684e1f7-26ac-319a-9de0-0a77791276e6/

U2 - 10.1194/jlr.D026260

DO - 10.1194/jlr.D026260

M3 - Journal articles

C2 - 22829652

VL - 53

SP - 2226

EP - 2230

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 1539-7262

IS - 10

ER -